Introduction of foreign genes into tomato protoplasts by electroporation

Mikako Tsukada, Tomonobu Kusano, Yoshichika Kitagawa

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    The conditions required for the electroporation of a plasmid which harbored the gene for chloramphenicol acetyltransferase (CAT) into protoplasts from cultivated tomato leaves were optimized at 666 or 7,000 V/cm (single discharge) using a 47-μF capacitor in the presence of 10-100 μg DNA. The CAT gene was strongly expressed by when under the control of the promoter for the 35S RNA from cauliflower mosaic virus and CAT activity was detected in cells 10 days after electroporation.

    Original languageEnglish
    Pages (from-to)599-603
    Number of pages5
    JournalPlant and Cell Physiology
    Volume30
    Issue number4
    DOIs
    Publication statusPublished - 1989 Jun

    Keywords

    • Chloramphenicol acetyltransferase gene
    • Cucumber mosaic virus RNA
    • Electroporation
    • Lycopersicon esculentum

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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