Introduction of foreign genes into tomato protoplasts by electroporation

Mikako Tsukada; Tomonobu Kusano; Yoshichika Kitagawa

doi:10.1093/oxfordjournals.pcp.a077781

Introduction of foreign genes into tomato protoplasts by electroporation

Mikako Tsukada, Tomonobu Kusano, Yoshichika Kitagawa

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The conditions required for the electroporation of a plasmid which harbored the gene for chloramphenicol acetyltransferase (CAT) into protoplasts from cultivated tomato leaves were optimized at 666 or 7,000 V/cm (single discharge) using a 47-μF capacitor in the presence of 10-100 μg DNA. The CAT gene was strongly expressed by when under the control of the promoter for the 35S RNA from cauliflower mosaic virus and CAT activity was detected in cells 10 days after electroporation.

Original language	English
Pages (from-to)	599-603
Number of pages	5
Journal	Plant and Cell Physiology
Volume	30
Issue number	4
DOIs	https://doi.org/10.1093/oxfordjournals.pcp.a077781
Publication status	Published - 1989 Jun

Keywords

Chloramphenicol acetyltransferase gene
Cucumber mosaic virus RNA
Electroporation
Lycopersicon esculentum

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

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10.1093/oxfordjournals.pcp.a077781

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title = "Introduction of foreign genes into tomato protoplasts by electroporation",

abstract = "The conditions required for the electroporation of a plasmid which harbored the gene for chloramphenicol acetyltransferase (CAT) into protoplasts from cultivated tomato leaves were optimized at 666 or 7,000 V/cm (single discharge) using a 47-μF capacitor in the presence of 10-100 μg DNA. The CAT gene was strongly expressed by when under the control of the promoter for the 35S RNA from cauliflower mosaic virus and CAT activity was detected in cells 10 days after electroporation.",

keywords = "Chloramphenicol acetyltransferase gene, Cucumber mosaic virus RNA, Electroporation, Lycopersicon esculentum",

author = "Mikako Tsukada and Tomonobu Kusano and Yoshichika Kitagawa",

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AU - Tsukada, Mikako

AU - Kusano, Tomonobu

AU - Kitagawa, Yoshichika

PY - 1989/6

Y1 - 1989/6

N2 - The conditions required for the electroporation of a plasmid which harbored the gene for chloramphenicol acetyltransferase (CAT) into protoplasts from cultivated tomato leaves were optimized at 666 or 7,000 V/cm (single discharge) using a 47-μF capacitor in the presence of 10-100 μg DNA. The CAT gene was strongly expressed by when under the control of the promoter for the 35S RNA from cauliflower mosaic virus and CAT activity was detected in cells 10 days after electroporation.

AB - The conditions required for the electroporation of a plasmid which harbored the gene for chloramphenicol acetyltransferase (CAT) into protoplasts from cultivated tomato leaves were optimized at 666 or 7,000 V/cm (single discharge) using a 47-μF capacitor in the presence of 10-100 μg DNA. The CAT gene was strongly expressed by when under the control of the promoter for the 35S RNA from cauliflower mosaic virus and CAT activity was detected in cells 10 days after electroporation.

KW - Chloramphenicol acetyltransferase gene

KW - Cucumber mosaic virus RNA

KW - Electroporation

KW - Lycopersicon esculentum

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ER -

Introduction of foreign genes into tomato protoplasts by electroporation

Abstract

Keywords

ASJC Scopus subject areas

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