Introduction of dna into chick embryos by in ovo electroporation

Harukazu Nakamura, Junichi Funahashi

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Gene transfer by in ovo electroporation has been applied to the study of developmental biology, especially to central nervous system (CNS) development. Plasmids are injected into the neural tube of stage 10 chick embryos, and a 25-V 25-msec square pulse is applied five times. Since DNA moves toward the anode, the cathode side of the neural tube is transfected, and the cathode side is used as the control. Expression of translation product of the introduced DNA is observed 2 h after electroporation, peaks around 20 h after electroporation and then weakens. Expression is transient when plasmids are used as expression vectors, but they are very suitable for studying early developmental events (e.g., gene expression cascades or interactions). Misexpression of Pax-5 is shown as an example.

Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalMethods
Volume24
Issue number1
DOIs
Publication statusPublished - 2001 Jan 1

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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