Intrinsically disordered region of influenza A NP regulates viral genome packaging via interactions with viral RNA and host PI(4,5)P2

Michinori Kakisaka, Kazunori Yamada, Akiko Yamaji-Hasegawa, Toshihide Kobayashi, Yoko Aida

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

To be incorporated into progeny virions, the viral genome must be transported to the inner leaflet of the plasma membrane (PM) and accumulate there. Some viruses utilize lipid components to assemble at the PM. For example, simian virus 40 (SV40) targets the ganglioside GM1 and human immunodeficiency virus type 1 (HIV-1) utilizes phosphatidylinositol (4,5) bisphosphate [PI(4,5)P2]. Recent studies clearly indicate that Rab11-mediated recycling endosomes are required for influenza A virus (IAV) trafficking of vRNPs to the PM but it remains unclear how IAV vRNP localized or accumulate underneath the PM for viral genome incorporation into progeny virions. In this study, we found that the second intrinsically disordered region (IDR2) of NP regulates two binding steps involved in viral genome packaging. First, IDR2 facilitates NP oligomer binding to viral RNA to form vRNP. Secondly, vRNP assemble by interacting with PI(4,5)P2 at the PM via IDR2. These findings suggest that PI(4,5)P2 functions as the determinant of vRNP accumulation at the PM.

Original languageEnglish
Pages (from-to)116-126
Number of pages11
JournalVirology
Volume496
DOIs
Publication statusPublished - 2016 Sep 1
Externally publishedYes

Keywords

  • Genome packaging
  • IAV
  • IDR
  • NP
  • PI(4,5)P2
  • VRNA

ASJC Scopus subject areas

  • Virology

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