Intravitreal injection of peanut agglutinin results in specific labeling of long-wave-length-sensitive cone photoreceptors

Makoto Ishikawa, Takeshi Yoshitomi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Purpose. To investigate whether intravitreally injected fluorescein (FITC)-conjugated peanut agglutinin (PNA) can be specifically bound to long-wavelength-sensitive cone photoreceptor outer segment membrane. Methods.Two microliters of FITC-PNA (100μg/ml) was intravitreally injected into dace. After two hours, the retinas were removed, and mounted on the slide glass. To isolate the PNA-positive photoreceptor cells, the flat mount retinal preparations were mechanically compressed under the cover glass. Specificity of PNA-labeling was examined by simultaneous flurescence and Nomarsky interference microscope. Results. By the application of intravitreal injection of FITC-PNA, the flat mount preparation showed regular arrangement of cone mosaic. The mechanical isolation procedure revealed that the outer segment of the long-double-cone and the long-single-cone photoreceptor cells were fluorescent positive. Conclusions.Intravitreally injected FITC-PNA results in specific labeling of outer segment of the long-wavelength-sensitive cone photoreceptors in vivo.Our results provide an efficient method for quantitative analysis of cone mosaic patterning.

Original languageEnglish
Title of host publicationPhotoreceptors
Subtitle of host publicationPhysiology, Types and Abnormalities
PublisherNova Science Publishers, Inc.
Pages163-169
Number of pages7
ISBN (Print)9781619426191
Publication statusPublished - 2012 Jul
Externally publishedYes

Keywords

  • Cell isolation
  • Cone photoreceptor cell
  • Dace (Tribolodonhakonensis)
  • Intravitreal injection
  • Long-wavelength sensitivity
  • Peanut agglutinin labeling

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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