Intravenously injected FK506 failed to inhibit hippocampal calcineurin

Motohiro Morioka; Kohji Fukunaga; Yutaka Kai; Tatemi Todaka; Shigetoshi Yano; Jun ichiro Hamada; Eishichi Miyamoto; Yukitaka Ushio

doi:10.1006/bbrc.2001.5468

Intravenously injected FK506 failed to inhibit hippocampal calcineurin

Motohiro Morioka, Kohji Fukunaga, Yutaka Kai, Tatemi Todaka, Shigetoshi Yano, Jun ichiro Hamada, Eishichi Miyamoto, Yukitaka Ushio

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

FK506 (tacrolimus) is known as an inhibitor for calcineurin and is used in numerous research fields. It is not clear whether intravenously injected FK506 inhibits neuronal calcineurin. We measured the calcineurin activities of normal and postischemic rat hippocampi after intravenous injection of FK506 (3 mg/kg). Intravenously injected FK506 had no inhibitory effect on calcineurin activity in the hippocampi of normal and postischemic rats, whereas FK506 inhibited the calcineurin in vitro (purified enzyme, hippocampal homogenate, and hippocampal slice culture homogenate). Thus, it is considered that intravenously injected FK506 does not act on intraneuronal calcineurin and that several effects of FK506 are not due to the inhibition of neuronal calcineurin.

Original language	English
Pages (from-to)	802-806
Number of pages	5
Journal	Biochemical and biophysical research communications
Volume	286
Issue number	4
DOIs	https://doi.org/10.1006/bbrc.2001.5468
Publication status	Published - 2001
Externally published	Yes

Keywords

Calcineurin
FK506
Hippocampus

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.2001.5468

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title = "Intravenously injected FK506 failed to inhibit hippocampal calcineurin",

abstract = "FK506 (tacrolimus) is known as an inhibitor for calcineurin and is used in numerous research fields. It is not clear whether intravenously injected FK506 inhibits neuronal calcineurin. We measured the calcineurin activities of normal and postischemic rat hippocampi after intravenous injection of FK506 (3 mg/kg). Intravenously injected FK506 had no inhibitory effect on calcineurin activity in the hippocampi of normal and postischemic rats, whereas FK506 inhibited the calcineurin in vitro (purified enzyme, hippocampal homogenate, and hippocampal slice culture homogenate). Thus, it is considered that intravenously injected FK506 does not act on intraneuronal calcineurin and that several effects of FK506 are not due to the inhibition of neuronal calcineurin.",

keywords = "Calcineurin, FK506, Hippocampus",

author = "Motohiro Morioka and Kohji Fukunaga and Yutaka Kai and Tatemi Todaka and Shigetoshi Yano and Hamada, {Jun ichiro} and Eishichi Miyamoto and Yukitaka Ushio",

note = "Funding Information: This work was supported by grants-in-aid for Scientific Research from the Ministry of Education, Sports, Science and Culture of Japan. We thank Ms. Ikuyo Ishimatsu for her technical assistance.",

year = "2001",

doi = "10.1006/bbrc.2001.5468",

language = "English",

volume = "286",

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T1 - Intravenously injected FK506 failed to inhibit hippocampal calcineurin

AU - Morioka, Motohiro

AU - Fukunaga, Kohji

AU - Kai, Yutaka

AU - Todaka, Tatemi

AU - Yano, Shigetoshi

AU - Hamada, Jun ichiro

AU - Miyamoto, Eishichi

AU - Ushio, Yukitaka

N1 - Funding Information: This work was supported by grants-in-aid for Scientific Research from the Ministry of Education, Sports, Science and Culture of Japan. We thank Ms. Ikuyo Ishimatsu for her technical assistance.

PY - 2001

Y1 - 2001

N2 - FK506 (tacrolimus) is known as an inhibitor for calcineurin and is used in numerous research fields. It is not clear whether intravenously injected FK506 inhibits neuronal calcineurin. We measured the calcineurin activities of normal and postischemic rat hippocampi after intravenous injection of FK506 (3 mg/kg). Intravenously injected FK506 had no inhibitory effect on calcineurin activity in the hippocampi of normal and postischemic rats, whereas FK506 inhibited the calcineurin in vitro (purified enzyme, hippocampal homogenate, and hippocampal slice culture homogenate). Thus, it is considered that intravenously injected FK506 does not act on intraneuronal calcineurin and that several effects of FK506 are not due to the inhibition of neuronal calcineurin.

AB - FK506 (tacrolimus) is known as an inhibitor for calcineurin and is used in numerous research fields. It is not clear whether intravenously injected FK506 inhibits neuronal calcineurin. We measured the calcineurin activities of normal and postischemic rat hippocampi after intravenous injection of FK506 (3 mg/kg). Intravenously injected FK506 had no inhibitory effect on calcineurin activity in the hippocampi of normal and postischemic rats, whereas FK506 inhibited the calcineurin in vitro (purified enzyme, hippocampal homogenate, and hippocampal slice culture homogenate). Thus, it is considered that intravenously injected FK506 does not act on intraneuronal calcineurin and that several effects of FK506 are not due to the inhibition of neuronal calcineurin.

KW - Calcineurin

KW - FK506

KW - Hippocampus

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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Intravenously injected FK506 failed to inhibit hippocampal calcineurin

Abstract

Keywords

ASJC Scopus subject areas

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