Intradermal measurement of reactive oxygen species using open-tip porous microneedles

Yuina Abe, Shuhei Yamaguchi, Hiroya Abe, Ryohei Takizawa, Yuki Tatsui, Matsuhiko Nishizawa

Research output: Contribution to journalArticlepeer-review

Abstract

Reactive oxygen species (ROS), which constitute a representative group of substances associated with skin health, are promising indicators of oxidative stress. A local electrochemical system can measure ROS with minimal invasiveness. In this study, a microneedle-based amperometric sensor is developed for real-time monitoring of changes in the H2O2 concentration of the skin. Two electrodes can be mounted on a single open-tip porous microneedle (PMN), which allows local measurement without intradermal noise caused by the high resistance and non-uniform distribution of the potential difference. The PMN electrode shows a current response in accordance with the H2O2 concentration, and amperometric measurement of ex vivo skin samples is successfully performed. The results indicate that a compact system based on a single needle is a promising tool for intracutaneous sensing of biological signals.

Original languageEnglish
Article number111877
JournalMicroelectronic Engineering
Volume265
DOIs
Publication statusPublished - 2022 Sept 15

Keywords

  • Biosensing
  • Electrochemical sensor
  • Microelectrode
  • Microneedle
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Electrical and Electronic Engineering

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