Intracolonic Administration of the TRPA1 Agonist Allyl Isothiocyanate Stimulates Colonic Motility and Defecation in Conscious Dogs

Soutoku Someya, Munenori Nagao, Chikashi Shibata, Naoki Tanaka, Hiroyuki Sasaki, Daisuke Kikuchi, Tomohiro Miyachi, Takeshi Naitoh, Michiaki Unno

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Background: The aim of the present study was to investigate the effects of the intracolonic transient receptor potential (TRP) A1 agonist allyl isothiocyanate (AITC) on colonic motility and defecation. Methods: The effects of AITC administered into the proximal colonic lumen on colonic motility and defecation were studied in neurally intact dogs equipped with strain-gauge force transducers on the colon, with or without various antagonists. Effects of intracolonic AITC were also studied in dogs with either transection/re-anastomosis (T/R) between the proximal and middle colon and complete extrinsic denervation of an ileocolonic segment. Results: AITC increased colonic motility and induced giant migrating contractions (GMCs) with defecations in 75 % of experiments in neurally intact dogs. These effects were inhibited by atropine, hexamethonium, ondansetron, and HC-030031 but unaltered by capsazepine. In dogs with T/R, the increase in colonic motility was inhibited in the middle-distal colon. In dogs with extrinsic denervation, the increase in colonic motility in the distal colon was decreased. Conclusions: Intracolonic AITC stimulates colonic motility and defecation via cholinergic, serotonergic, and TRPA1 pathways. Continuity of colonic enteric neurons plays an essential role in the intracolonic AITC-induced colonic motor response, while extrinsic nerves are important in occurrence and propagation of GMCs.

Original languageEnglish
Pages (from-to)1342-1349
Number of pages8
JournalJournal of Gastrointestinal Surgery
Volume19
Issue number7
DOIs
Publication statusPublished - 2015 Jul 19

Keywords

  • Allyl isothiocyanate
  • Giant migrating contractions
  • TRPA1

ASJC Scopus subject areas

  • Surgery
  • Gastroenterology

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