TY - JOUR
T1 - Intracolonic Administration of the TRPA1 Agonist Allyl Isothiocyanate Stimulates Colonic Motility and Defecation in Conscious Dogs
AU - Someya, Soutoku
AU - Nagao, Munenori
AU - Shibata, Chikashi
AU - Tanaka, Naoki
AU - Sasaki, Hiroyuki
AU - Kikuchi, Daisuke
AU - Miyachi, Tomohiro
AU - Naito, Takeshi
AU - Unno, Michiaki
N1 - Funding Information:
This work was supported by Grant-In-Aid 23591953 from the Japan Society for the Promotion of Science.
Publisher Copyright:
© 2015, The Author(s).
PY - 2015/7/19
Y1 - 2015/7/19
N2 - Background: The aim of the present study was to investigate the effects of the intracolonic transient receptor potential (TRP) A1 agonist allyl isothiocyanate (AITC) on colonic motility and defecation. Methods: The effects of AITC administered into the proximal colonic lumen on colonic motility and defecation were studied in neurally intact dogs equipped with strain-gauge force transducers on the colon, with or without various antagonists. Effects of intracolonic AITC were also studied in dogs with either transection/re-anastomosis (T/R) between the proximal and middle colon and complete extrinsic denervation of an ileocolonic segment. Results: AITC increased colonic motility and induced giant migrating contractions (GMCs) with defecations in 75 % of experiments in neurally intact dogs. These effects were inhibited by atropine, hexamethonium, ondansetron, and HC-030031 but unaltered by capsazepine. In dogs with T/R, the increase in colonic motility was inhibited in the middle-distal colon. In dogs with extrinsic denervation, the increase in colonic motility in the distal colon was decreased. Conclusions: Intracolonic AITC stimulates colonic motility and defecation via cholinergic, serotonergic, and TRPA1 pathways. Continuity of colonic enteric neurons plays an essential role in the intracolonic AITC-induced colonic motor response, while extrinsic nerves are important in occurrence and propagation of GMCs.
AB - Background: The aim of the present study was to investigate the effects of the intracolonic transient receptor potential (TRP) A1 agonist allyl isothiocyanate (AITC) on colonic motility and defecation. Methods: The effects of AITC administered into the proximal colonic lumen on colonic motility and defecation were studied in neurally intact dogs equipped with strain-gauge force transducers on the colon, with or without various antagonists. Effects of intracolonic AITC were also studied in dogs with either transection/re-anastomosis (T/R) between the proximal and middle colon and complete extrinsic denervation of an ileocolonic segment. Results: AITC increased colonic motility and induced giant migrating contractions (GMCs) with defecations in 75 % of experiments in neurally intact dogs. These effects were inhibited by atropine, hexamethonium, ondansetron, and HC-030031 but unaltered by capsazepine. In dogs with T/R, the increase in colonic motility was inhibited in the middle-distal colon. In dogs with extrinsic denervation, the increase in colonic motility in the distal colon was decreased. Conclusions: Intracolonic AITC stimulates colonic motility and defecation via cholinergic, serotonergic, and TRPA1 pathways. Continuity of colonic enteric neurons plays an essential role in the intracolonic AITC-induced colonic motor response, while extrinsic nerves are important in occurrence and propagation of GMCs.
KW - Allyl isothiocyanate
KW - Giant migrating contractions
KW - TRPA1
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U2 - 10.1007/s11605-015-2813-4
DO - 10.1007/s11605-015-2813-4
M3 - Article
C2 - 25855335
AN - SCOPUS:84931564951
VL - 19
SP - 1342
EP - 1349
JO - Journal of Gastrointestinal Surgery
JF - Journal of Gastrointestinal Surgery
SN - 1091-255X
IS - 7
ER -