Intracellular Ca2+ imaging for micropatterned cardiac myocytes

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The patterning of cardiac myocytes on a micron scale (-5 pm) was achieved by microcontact printing of fibronectin onto a hydrophobically pretreated glass substrate. The patterned cardiac myocytes conjugated with each other by forming a gap junction, as judged from the synchronized Ca2+ transition over the pattern, and thus simultaneously contracted. The dynamic change of the Ca2+ concentration within the patterned tissue was analyzed quantitatively during successive contraction and relaxation using a Nipkow-type high-speed confocal microscope.

Original languageEnglish
Pages (from-to)748-751
Number of pages4
JournalBiotechnology and Bioengineering
Volume81
Issue number6
DOIs
Publication statusPublished - 2003 Mar 20

Keywords

  • Ca imaging
  • Cardiac myocytes
  • Confocal microscope
  • Microcontact printing (pCP)
  • Pattering

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Fingerprint Dive into the research topics of 'Intracellular Ca2+ imaging for micropatterned cardiac myocytes'. Together they form a unique fingerprint.

  • Cite this