Intracellular calcium concentration of acinar cells in feline tracheal submucosal glands

H. Ishihara, S. Shimura, M. Sato, T. Masuda, N. Ishide, M. Miura, T. Sasaki, H. Sasaki, T. Takishima

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We measured the intracellular free calcium ion concentration ([Ca2+](i)) of acinar cells in isolated feline tracheal submucosal glands in response to secretagogues using the Ca2+-sensitive fluorescent dye fura-2. The secretagogues included cholinergic, adrenergic agonists, substance P (SP), and vasoactive intestinal polypeptide (VIP) which induce mucus glycoprotein secretion from feline tracheal submucosal glands. Methacholine (MCh) produced a significant increase in [Ca2+](i) of up to 9.8 times that of control in a dose-dependent fashion at concentrations of 10-8 to 10-3 M. [Ca2+](i) increase by MCh reached a peak within 30 s after stimulation and thereafter showed a sustained rise. In Ca2+-free medium, MCh produced an initial transient rise, which was <30% of that in a Ca2+-containing solution, and which lasted for 60 s with no prolonged sustained rise in [Ca2+](i). Atropine abolished MCh-evoked [Ca2+](i) increase. Phenylephrine and SP produced a prolonged increase in [Ca2+](i) without an initial transient increase. Phenylephrine (up to 10-4 M) evoked an increase in [Ca2+](i) by up to 240% that of control, which was abolished by prazosin. SP (up to 10-4 M) also evoked an increase in [Ca2+](i) by 155% that of control, which was abolished by atropine. By contrast, both isoproterenol (up to 10-5 M) and VIP (up to 10-5 M) failed to alter [Ca2+](i). These findings indicate that the mucus glycoprotein secretion evoked by muscarinic cholinergic, α-adrenergic agonist or SP can be mediated by intracellular Ca2+, whereas that by β-adrenergic agonists or VIP cannot.

Original languageEnglish
Pages (from-to)L345-L350
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume259
Issue number6 3-3
DOIs
Publication statusPublished - 1990

Keywords

  • cat, intracellular calcium ion
  • cholinergic agonist, α-adrenergic agonist
  • substance P
  • tracheal submucosal gland

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Fingerprint Dive into the research topics of 'Intracellular calcium concentration of acinar cells in feline tracheal submucosal glands'. Together they form a unique fingerprint.

  • Cite this