TY - GEN
T1 - Interspecific hybrids between asparagus schoberioides kunth and A. officinalis L.
AU - Ochiai, T.
AU - Kanno, A.
AU - Kameya, T.
AU - Sonoda, T.
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Interspecific hybrids are very important breeding materials to introduce new genes from wild species into crop plants. In this study, A. officinalis (2n=2x=20) cv. Mary Washington 500W was crossed with A. schoberioides (2n=4x=40), which is a dioecious species distributed in China, Korea and Japan. We obtained putative interspecific hybrid plants from reciprocal crosses between these two species. Five F1 progenies (A. schoberioides × A. officinalis) were used for cytological and RFLP analyses. The chromosome number of all F1 progenies was 2n=3x=30. The RFLP patterns of chloroplast DNA in the progenies were the same as the pattern of maternal parent, A. schoberioides. Additionally, the RFLP patterns of ribosomal DNA in the progenies shared the fragments specific for both parents. These results suggest that the cytoplasm of putative hybrid plants was derived from the maternal parent and that nuclear DNA of the plant was derived from both parents, showing that these progenies were interspecific hybrids between A. schoberioides and A. officinalis. The morphological characters of five hybrid plants appeared to be mixtures of both parents. Out of five plants, three plants flowered; one was male and two were females. This indicates that these two species seem to have the same mechanism of sex determination.
AB - Interspecific hybrids are very important breeding materials to introduce new genes from wild species into crop plants. In this study, A. officinalis (2n=2x=20) cv. Mary Washington 500W was crossed with A. schoberioides (2n=4x=40), which is a dioecious species distributed in China, Korea and Japan. We obtained putative interspecific hybrid plants from reciprocal crosses between these two species. Five F1 progenies (A. schoberioides × A. officinalis) were used for cytological and RFLP analyses. The chromosome number of all F1 progenies was 2n=3x=30. The RFLP patterns of chloroplast DNA in the progenies were the same as the pattern of maternal parent, A. schoberioides. Additionally, the RFLP patterns of ribosomal DNA in the progenies shared the fragments specific for both parents. These results suggest that the cytoplasm of putative hybrid plants was derived from the maternal parent and that nuclear DNA of the plant was derived from both parents, showing that these progenies were interspecific hybrids between A. schoberioides and A. officinalis. The morphological characters of five hybrid plants appeared to be mixtures of both parents. Out of five plants, three plants flowered; one was male and two were females. This indicates that these two species seem to have the same mechanism of sex determination.
KW - Asparagus officinalis
KW - Asparagus schoberioides
KW - Interspecific hybrids
KW - Mechanism of sex determination
KW - Reciprocal cross
UR - http://www.scopus.com/inward/record.url?scp=33645478440&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645478440&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2002.589.31
DO - 10.17660/ActaHortic.2002.589.31
M3 - Conference contribution
AN - SCOPUS:33645478440
SN - 9789066057968
T3 - Acta Horticulturae
SP - 225
EP - 229
BT - X International Asparagus Symposium
PB - International Society for Horticultural Science
ER -