Interleukin-17 augments tumor necrosis factor-α-induced elaboration of proangiogenic factors from fibroblasts

Muneo Numasaki, Michael T. Lotze, Hidetada Sasaki

Research output: Contribution to journalArticlepeer-review

110 Citations (Scopus)


Interleukin-17 (IL-17) is a CD4 T cell cytokine. In this report, we investigated the effects of this cytokine on the elaboration of proangiogenic factors by lung fibroblasts. After stimulation with a wide range of doses of IL-17, fibroblasts produced more amount of various kinds of angiogenic factors including NO, HGF, MCP-1, KC, MIP-2, PGE1, PGE2 and VEGF in a dose-dependent manner. Treatment with a COX-1 and COX-2 inhibitor indomethacin did not impair IL-17-induced HGF and VEGF secretion in fibroblasts. In addition, TNF-α alone stimulated the elaboration of KC, MIP-2, PGE2 and VEGF in fibroblasts. IL-17 and TNF-α in combination up-regulated elaboration of these proangiogenic factors additively or synergistically. Moreover, conditioned media (CM) from IL-17-stimulated fibroblasts showed significantly higher activity on endothelial cell growth than those from non-treated control cells. These results indicate that IL-17 up-regulates elaboration of various proangiogenic factors, and modulates macrophage-derived TNF-α-induced production of KC, MIP-2, PGE2 and VEGF by fibroblasts. Our findings also demonstrate that IL-17 might be a potential contributor to the inflammatory angiogenesis via induction of proangiogenic factors by stromal fibroblasts.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalImmunology Letters
Issue number1
Publication statusPublished - 2004 Apr 30


  • EC
  • HGF
  • IL
  • KC
  • MCP
  • MIP-2
  • NO
  • PGE
  • TNF
  • VEGF
  • endothelial cells
  • hepatocyte growth factor
  • interleukin
  • keratinocyte-derived chemokine
  • macrophage inflammatory protein-2
  • monocyte chemoattractant protein
  • nitric oxide
  • prostaglandin E
  • tumor necrosis factor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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