Abstract
Interleukin-17 (IL-17) is a CD4 T cell cytokine. In this report, we investigated the effects of this cytokine on the elaboration of proangiogenic factors by lung fibroblasts. After stimulation with a wide range of doses of IL-17, fibroblasts produced more amount of various kinds of angiogenic factors including NO, HGF, MCP-1, KC, MIP-2, PGE1, PGE2 and VEGF in a dose-dependent manner. Treatment with a COX-1 and COX-2 inhibitor indomethacin did not impair IL-17-induced HGF and VEGF secretion in fibroblasts. In addition, TNF-α alone stimulated the elaboration of KC, MIP-2, PGE2 and VEGF in fibroblasts. IL-17 and TNF-α in combination up-regulated elaboration of these proangiogenic factors additively or synergistically. Moreover, conditioned media (CM) from IL-17-stimulated fibroblasts showed significantly higher activity on endothelial cell growth than those from non-treated control cells. These results indicate that IL-17 up-regulates elaboration of various proangiogenic factors, and modulates macrophage-derived TNF-α-induced production of KC, MIP-2, PGE2 and VEGF by fibroblasts. Our findings also demonstrate that IL-17 might be a potential contributor to the inflammatory angiogenesis via induction of proangiogenic factors by stromal fibroblasts.
Original language | English |
---|---|
Pages (from-to) | 39-43 |
Number of pages | 5 |
Journal | Immunology Letters |
Volume | 93 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2004 Apr 30 |
Keywords
- EC
- HGF
- IL
- KC
- MCP
- MIP-2
- NO
- PGE
- TNF
- VEGF
- endothelial cells
- hepatocyte growth factor
- interleukin
- keratinocyte-derived chemokine
- macrophage inflammatory protein-2
- monocyte chemoattractant protein
- nitric oxide
- prostaglandin E
- tumor necrosis factor
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology