TY - JOUR
T1 - Interleukin-12 prevents diaphragm muscle deterioration in a septic animal model
AU - Nakahata, Eri
AU - Shindoh, Yuriko
AU - Takayama, Tetsuro
AU - Shindoh, Chiyohiko
N1 - Funding Information:
The authors wish to thank Thomas Mandeville for his review of the English in this paper. This study was supported in part, by a grant from the Ministry of Education, Science, Sports and Culture of Japan (no. 12670545).
PY - 2001
Y1 - 2001
N2 - The effects of an intravenous injection of Interleukin-12 (IL-12) after endotoxin administration and without endotoxin administration on diaphragm muscle were studied using Wistar rats. Three treatment groups, namely a control (Saline + endotoxin) group, an IL-12 + endotoxin group and an IL-12 only group were studied. E. coli endotoxin (30 mg/kg) was injected intraperitoneally 5 min after Saline or IL-12 (0.25 μg) injection. In the control group, the force-frequency curves, twitch tension (TT) and slope during contraction time (TT/CT) were significantly lower at 4 h than those at 0 h due to endotoxin (P < 0.001, P < 0.01 and P < 0.01, respectively), and NO production was increased at 4 h as shown by NADPH diaphorase staining. In the IL-12 + endotoxin group, the decrement of the force-frequency curves, TT and TT/CT induced by endotoxin at 4 h were significantly prevented compared with those of the control group (P < 0.001, P < 0.05 and P < 0.05, respectively), and NO production was blocked at 4 h. In the IL-12 only group, the force-frequency curves were decreased in the range of high frequency and IL-12 resulted in NO production. Furthermore, the positive muscle fibers detected by NADPH diaphorase staining were classified as type I and IIa muscle fibers by ATPase staining in the control and IL-12 only groups. It is concluded that IL-12 prevents the deterioration of diaphragm muscle contraction induced by endotoxin by reducing NO production in type I and IIa muscle fibers. These results suggest that IL-12 and endotoxin may interfere with each other.
AB - The effects of an intravenous injection of Interleukin-12 (IL-12) after endotoxin administration and without endotoxin administration on diaphragm muscle were studied using Wistar rats. Three treatment groups, namely a control (Saline + endotoxin) group, an IL-12 + endotoxin group and an IL-12 only group were studied. E. coli endotoxin (30 mg/kg) was injected intraperitoneally 5 min after Saline or IL-12 (0.25 μg) injection. In the control group, the force-frequency curves, twitch tension (TT) and slope during contraction time (TT/CT) were significantly lower at 4 h than those at 0 h due to endotoxin (P < 0.001, P < 0.01 and P < 0.01, respectively), and NO production was increased at 4 h as shown by NADPH diaphorase staining. In the IL-12 + endotoxin group, the decrement of the force-frequency curves, TT and TT/CT induced by endotoxin at 4 h were significantly prevented compared with those of the control group (P < 0.001, P < 0.05 and P < 0.05, respectively), and NO production was blocked at 4 h. In the IL-12 only group, the force-frequency curves were decreased in the range of high frequency and IL-12 resulted in NO production. Furthermore, the positive muscle fibers detected by NADPH diaphorase staining were classified as type I and IIa muscle fibers by ATPase staining in the control and IL-12 only groups. It is concluded that IL-12 prevents the deterioration of diaphragm muscle contraction induced by endotoxin by reducing NO production in type I and IIa muscle fibers. These results suggest that IL-12 and endotoxin may interfere with each other.
KW - ATPase
KW - Cytokine
KW - Endotoxin
KW - Free radicals
KW - NADPH diaphorase
KW - Nitric oxide
KW - Skeletal muscle
KW - Superoxide
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U2 - 10.1016/S1095-6433(01)00396-8
DO - 10.1016/S1095-6433(01)00396-8
M3 - Article
C2 - 11691602
AN - SCOPUS:0034747938
VL - 130
SP - 653
EP - 663
JO - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
SN - 1095-6433
IS - 4
ER -