Interleukin 1 gene expression in adult T cell leukemia

Y. Wano, T. Hattori, M. Matsuoka, K. Takatsuki, A. O. Chua, U. Gubler, W. C. Greene

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126 Citations (Scopus)


The adult T cell leukemia (ATL) is a T cell neoplasm etiologically associated with human T lymphotropic virus type I (HTLV-I) infection. ATL cells often abnormally express interleukin 2 (IL-2) receptors, and ATL patients may show clinical evidence of hypercalcemia, osteolytic bone lesions, or increased bone turnover. Whereas interleukin 1 (IL-1) is not generally recognized as a product of T cells, this cytokine is capable of both altering IL-2 receptor expression and activating osteoclasts. Thus, we investigated the possibility that primary ATL leukemic T cells and HTLV-I-infected long-term ATL cell lines produce IL-1. S1 nuclease protection assays demonstrated that primary leukemic ATL cells from five out of six patients, as well as one patient with T4+ chronic lymphocytic leukemia, contained considerable quantities of IL-1β messenger RNA (mRNA) and small amounts of IL-1α mRNA. These primary leukemic T cells also released biologically active IL-1 protein as evaluated in the murine thymocyte comitogenesis bioassay. In contrast to primary tumor cells, four out of six long-term ATL cell lines produced variable amounts of IL-1α mRNA in the absence of detectable IL-1β mRNA as measured by S1 nuclease protection. These data demonstrate that IL-1 gene (especially IL-1β) expression occurs in many primary HTLV-I-infected leukemic T cells raising the possibility that this mediator may play a role in the pathological changes associated with this leukemia. Also, these studies show that the pattern of IL-1α and IL-1β gene expression differs between primary ATL tumor cells and long-term cultured ATL cell lines, indicating an interesting biological difference in these two HTLV-I-infected cell populations.

Original languageEnglish
Pages (from-to)911-916
Number of pages6
JournalJournal of Clinical Investigation
Issue number3
Publication statusPublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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