Interleukin-1β induces death in chondrocyte-like ATDC5 cells through mitochondrial dysfunction and energy depletion in a reactive nitrogen and oxygen species-dependent manner

Rika Yasuhara, Yoichi Miyamoto, Takaaki Akaike, Teruo Akuta, Masanori Nakamura, Masamichi Takami, Naoko Morimura, Kayoko Yasu, Ryutaro Kamijo

Research output: Contribution to journalArticlepeer-review

80 Citations (Scopus)

Abstract

IL-1 (interleukin-1) acts as a key mediator of the degeneration of articular cartilage in RA (rheumatoid arthritis) and OA (osteoarthritis), where chondrocyte death is observed. It is still controversial, however, whether IL-1 induces chondrocyte death. In the present study, the viability of mouse chondrocyte-like ATDC5 cells was reduced by the treatment with IL-1β for 48 h or longer. IL-1β augmented the expression of the catalytic gp91 subunit of NADPH oxidase, gp91phox, as well as inducible NO synthase in ATDC5 cells. Generation of nitrated guanosine and tyrosine suggested the formation of reactive nitrogen species including ONOO- (peroxynitrite), a reaction product of NO and O2-, in ATDC5 cells and rat primary chondrocytes treated with IL-1β. Death of ATDC5 cells after IL-1β treatment was prevented by an NADPH-oxidase inhibitor, AEBSF [4-(2-aminoethyl)benzene-sulphonyl fluoride], an NO synthase inhibitor, L-NAME (NG-nitro-L-arginine methyl ester), and a ONOO- scavenger, uric acid. The viability of ATDC5 cells was reduced by the ONOO --generator 3-(4-morpholinyl)sydnonimine hydrochloride, but not by either the NO-donor 1-hydroxy-2-oxo-3-(N-methyl-2-amino-propyl)-3-methyl-1- triazene or 5-nitrosoglutathione. Disruption of mitochondrial membrane potential and ATP deprivation were observed in IL-1β-treated ATDC5 cells, both of which were restored by L-NAME, AEBSF or uric acid. On the other hand, no morphological or biochemical signs indicating apoptosis were observed in these cells. These results suggest that the death of chondrocyte-like ATDC5 cells was mediated at least in part by mitochondrial dysfunction and energy depletion through ONOO- formation after IL-1β treatment.

Original languageEnglish
Pages (from-to)315-323
Number of pages9
JournalBiochemical Journal
Volume389
Issue number2
DOIs
Publication statusPublished - 2005 Jul 15

Keywords

  • Interleukin-1β
  • NADPH oxidase
  • Nitric oxide
  • Nitric oxide synthase
  • Peroxynitrite
  • Superoxide

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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