TY - JOUR
T1 - Interindividual difference in expression of human ah receptor and related p450 genes
AU - Hayashi, Shin ichi
AU - Watanabe, Junko
AU - Nakachi, Kei
AU - Eguchi, Hidetaka
AU - Gotoh, Osamu
AU - Kawajiri, K. Kaname
N1 - Funding Information:
We are grateful to Dr Kazue Imai for her assistance in collection of Wood samples. This study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, a research grant from the Ministry of Health and Welfare of Japan, and Research Grant of the Princess Takamatsu Cancer Research Fund.
PY - 1994/5
Y1 - 1994/5
N2 - The genomic clones of human aryl hydrocarbon receptor (Ahr) and Ahr nuclear translocator (Arnt) were isolated, and the structures of exon-intron junctions of these genes were partially determined. Based on the sequence information, a quantitative RT-PCR analysis was developed, and the expression of these genes was studied in various human tissues. mRNAs for Ahr and Arnt were widely expressed in human tissues and abundantly in lung. Individual difference in expression levels of Ahr and Arnt mRNA was observed in liver, lung and blood. In order to examine whether expression levels of Ahr and Arnt were associated with those of CYP1A1, we studied the expression of these mRNAs in blood among 20 healthy subjects, taking account of individuals' cigarette smoking habits. We found that the expression levels of CYP1Al appeared to associate with those of Ahr and Arnt mRNAs (P ≤ 0.06), and also that the expression of Ahr and Arnt was influenced by cigarette smoking. The expression of human Ahr and Arnt is reported here for the first time, providing a quantitative RT-PCR analysis as a useful tool for further studies.
AB - The genomic clones of human aryl hydrocarbon receptor (Ahr) and Ahr nuclear translocator (Arnt) were isolated, and the structures of exon-intron junctions of these genes were partially determined. Based on the sequence information, a quantitative RT-PCR analysis was developed, and the expression of these genes was studied in various human tissues. mRNAs for Ahr and Arnt were widely expressed in human tissues and abundantly in lung. Individual difference in expression levels of Ahr and Arnt mRNA was observed in liver, lung and blood. In order to examine whether expression levels of Ahr and Arnt were associated with those of CYP1A1, we studied the expression of these mRNAs in blood among 20 healthy subjects, taking account of individuals' cigarette smoking habits. We found that the expression levels of CYP1Al appeared to associate with those of Ahr and Arnt mRNAs (P ≤ 0.06), and also that the expression of Ahr and Arnt was influenced by cigarette smoking. The expression of human Ahr and Arnt is reported here for the first time, providing a quantitative RT-PCR analysis as a useful tool for further studies.
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U2 - 10.1093/carcin/15.5.801
DO - 10.1093/carcin/15.5.801
M3 - Article
C2 - 7515333
AN - SCOPUS:0028239139
VL - 15
SP - 801
EP - 806
JO - Carcinogenesis
JF - Carcinogenesis
SN - 0143-3334
IS - 5
ER -
