TY - JOUR
T1 - Interferon-induced differentiation of U937 cells. Comparison with other agents that promote differentiation of human myeloid or monocytelike cell lines
AU - Hattori, T.
AU - Pack, M.
AU - Bougnoux, P.
AU - Chang, Z. L.
AU - Hoffman, T.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1983
Y1 - 1983
N2 - Effects of human fibroblast (β) or leukocyte (α) interferon (IFN) on differentiations of a human histiocytic lymphoma-derived cell line (U937) or promyelocytic leukemia-derived cell line (HL-60) were studied. When cultured with β-IFN (400-1,000 U/ml). U937 cells showed gross morphologic and microscopic changes consisting of clumping, increased cytoplasmic-to-nuclear ratio, enhanced prominence of cytoplasmic granules, and membrane ruffling. After culture with β-IFN, the number of U937 cells reactive with B43.4.1 monoclonal antibody, which is specific for human monocytes, natural killer cells, and neutrophils, increased from <10% of U937 cells to 47%. β-IFN treatment also enhanced antibody-dependent cellular cytotoxicity against chicken erythrocytes by U937 cells. The same morphologic, phenotypic, and functional changes were also observed when U937 were treated with recombinant or natural α-IFN. The effects of α-IFN were totally abolished by anti-α-IFN serum. In contrast, HL-60, which differentiates toward cells of the monocyte lineage in response to phorbol 12-myristate 13-acetate (based on the above criteria), and toward granulocytes in response to dimethyl sulfoxide, did not differentiate when cultured with α- or β-IFN. No consistent relationship between induction of differentiation and changes in phospholipid methylation were observed.
AB - Effects of human fibroblast (β) or leukocyte (α) interferon (IFN) on differentiations of a human histiocytic lymphoma-derived cell line (U937) or promyelocytic leukemia-derived cell line (HL-60) were studied. When cultured with β-IFN (400-1,000 U/ml). U937 cells showed gross morphologic and microscopic changes consisting of clumping, increased cytoplasmic-to-nuclear ratio, enhanced prominence of cytoplasmic granules, and membrane ruffling. After culture with β-IFN, the number of U937 cells reactive with B43.4.1 monoclonal antibody, which is specific for human monocytes, natural killer cells, and neutrophils, increased from <10% of U937 cells to 47%. β-IFN treatment also enhanced antibody-dependent cellular cytotoxicity against chicken erythrocytes by U937 cells. The same morphologic, phenotypic, and functional changes were also observed when U937 were treated with recombinant or natural α-IFN. The effects of α-IFN were totally abolished by anti-α-IFN serum. In contrast, HL-60, which differentiates toward cells of the monocyte lineage in response to phorbol 12-myristate 13-acetate (based on the above criteria), and toward granulocytes in response to dimethyl sulfoxide, did not differentiate when cultured with α- or β-IFN. No consistent relationship between induction of differentiation and changes in phospholipid methylation were observed.
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U2 - 10.1172/JCI110962
DO - 10.1172/JCI110962
M3 - Article
C2 - 6348086
AN - SCOPUS:0020576472
VL - 72
SP - 237
EP - 244
JO - [No source information available]
JF - [No source information available]
IS - 1
ER -