HetNet is a promising next-generation network. One of the problems in HetNet, when macro-cell base station (MBS) and small-cell BSs (SBSs) share the same radio resource, is the co-channel interference (CCI) between cells, specifically the CCI between macro cell and small cell. Therefore, channel assignment for HetNet is an important issue. Recently the authors proposed interference-aware channel segregation based dynamic channel assignment (IACS-DCA), in which each BS periodically measures the CCI power on all available channels and selects the channel with the lowest average CCI power computed from the past measurements. The channel with the lowest average CCI power is considered not to be used by the neighboring BSs inside and outside of the macro cell of interest, and therefore, IACS-DCA forms a channel reuse pattern with low CCI in a distributed manner. In this paper, we apply IACS-DCA to HetNet using time- and frequency-division channels. We show by computer simulation that IACS-DCA can form a channel reuse pattern with low CCI using time- and frequency-division channels in a distributed manner.