Interdigitated μ-electrodes for development of an impedimetric immunosensor for atrazine detection

Enrique Valera; Javier Ramón-Azcón; F. J. Sanchez; M. P. Marco; Angel Rodríguez; Luis M. Castañer

doi:10.1109/SCED.2009.4800485

Interdigitated μ-electrodes for development of an impedimetric immunosensor for atrazine detection

Enrique Valera, Javier Ramón-Azcón, F. J. Sanchez, M. P. Marco, Angel Rodríguez, Luis M. Castañer

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This contribution describes the development of an impedimetric immunosensor for atrazine detection. This immunosensor is based on the use of interdigitated metallic p-electrodes (IDμEs). The method described in this work does not use any redox mediator and relies on the direct detection of the immunochemical competitive reaction between the pesticide and a haptenized-protein immobilized on interdigitated p-electrodes for the specific antibody. The immunoreagents used were specifically developed to detect atrazine. The immunochemical detection of this pesticide is achieved without using any label. The immunosensor shows a limit of detection of 8.34±1.37 pg L^-1, which is lower than the Maximum Residue Level (MRL) (50 μg L^-1) established by EU (European Union) for residues of atrazine as herbicide in the wine grapes and other foodstuff products.

Original language	English
Title of host publication	Proceedings of the 2009 Spanish Conference on Electron Devices, CDE'09
Pages	278-280
Number of pages	3
DOIs	https://doi.org/10.1109/SCED.2009.4800485
Publication status	Published - 2009
Externally published	Yes
Event	2009 Spanish Conference on Electron Devices, CDE'09 - Santiago de Compostela, Spain Duration: 2009 Feb 11 → 2009 Feb 13

Publication series

Name	Proceedings of the 2009 Spanish Conference on Electron Devices, CDE'09

Other

Other	2009 Spanish Conference on Electron Devices, CDE'09
Country	Spain
City	Santiago de Compostela
Period	09/2/11 → 09/2/13

ASJC Scopus subject areas

Computational Theory and Mathematics
Hardware and Architecture
Electrical and Electronic Engineering

Access to Document

10.1109/SCED.2009.4800485

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Cite this

Valera, E., Ramón-Azcón, J., Sanchez, F. J., Marco, M. P., Rodríguez, A., & Castañer, L. M. (2009). Interdigitated μ-electrodes for development of an impedimetric immunosensor for atrazine detection. In Proceedings of the 2009 Spanish Conference on Electron Devices, CDE'09 (pp. 278-280). [4800485] (Proceedings of the 2009 Spanish Conference on Electron Devices, CDE'09). https://doi.org/10.1109/SCED.2009.4800485

Interdigitated μ-electrodes for development of an impedimetric immunosensor for atrazine detection. / Valera, Enrique; Ramón-Azcón, Javier; Sanchez, F. J.; Marco, M. P.; Rodríguez, Angel; Castañer, Luis M.

Proceedings of the 2009 Spanish Conference on Electron Devices, CDE'09. 2009. p. 278-280 4800485 (Proceedings of the 2009 Spanish Conference on Electron Devices, CDE'09).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Valera, E, Ramón-Azcón, J, Sanchez, FJ, Marco, MP, Rodríguez, A & Castañer, LM 2009, Interdigitated μ-electrodes for development of an impedimetric immunosensor for atrazine detection. in Proceedings of the 2009 Spanish Conference on Electron Devices, CDE'09., 4800485, Proceedings of the 2009 Spanish Conference on Electron Devices, CDE'09, pp. 278-280, 2009 Spanish Conference on Electron Devices, CDE'09, Santiago de Compostela, Spain, 09/2/11. https://doi.org/10.1109/SCED.2009.4800485

Valera E, Ramón-Azcón J, Sanchez FJ, Marco MP, Rodríguez A, Castañer LM. Interdigitated μ-electrodes for development of an impedimetric immunosensor for atrazine detection. In Proceedings of the 2009 Spanish Conference on Electron Devices, CDE'09. 2009. p. 278-280. 4800485. (Proceedings of the 2009 Spanish Conference on Electron Devices, CDE'09). https://doi.org/10.1109/SCED.2009.4800485

Valera, Enrique ; Ramón-Azcón, Javier ; Sanchez, F. J. ; Marco, M. P. ; Rodríguez, Angel ; Castañer, Luis M. / Interdigitated μ-electrodes for development of an impedimetric immunosensor for atrazine detection. Proceedings of the 2009 Spanish Conference on Electron Devices, CDE'09. 2009. pp. 278-280 (Proceedings of the 2009 Spanish Conference on Electron Devices, CDE'09).

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abstract = "This contribution describes the development of an impedimetric immunosensor for atrazine detection. This immunosensor is based on the use of interdigitated metallic p-electrodes (IDμEs). The method described in this work does not use any redox mediator and relies on the direct detection of the immunochemical competitive reaction between the pesticide and a haptenized-protein immobilized on interdigitated p-electrodes for the specific antibody. The immunoreagents used were specifically developed to detect atrazine. The immunochemical detection of this pesticide is achieved without using any label. The immunosensor shows a limit of detection of 8.34±1.37 pg L-1, which is lower than the Maximum Residue Level (MRL) (50 μg L-1) established by EU (European Union) for residues of atrazine as herbicide in the wine grapes and other foodstuff products.",

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AB - This contribution describes the development of an impedimetric immunosensor for atrazine detection. This immunosensor is based on the use of interdigitated metallic p-electrodes (IDμEs). The method described in this work does not use any redox mediator and relies on the direct detection of the immunochemical competitive reaction between the pesticide and a haptenized-protein immobilized on interdigitated p-electrodes for the specific antibody. The immunoreagents used were specifically developed to detect atrazine. The immunochemical detection of this pesticide is achieved without using any label. The immunosensor shows a limit of detection of 8.34±1.37 pg L-1, which is lower than the Maximum Residue Level (MRL) (50 μg L-1) established by EU (European Union) for residues of atrazine as herbicide in the wine grapes and other foodstuff products.

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