Intercalating fluorescence dye YOYO-1 prevents the folding transition in giant duplex DNA

Natsuhiko Yoshinaga, Tatsuo Akitaya, Kenichi Yoshikawa

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Recently, it has become clear that with the addition of polyamines, giant DNA molecules of size greater than 10 kbp exhibit all-or-none switching between elongated coil and folded compact states. Here the effects of the intercalating fluorescent labeling dye, YOYO-1, and the minor-groove binding fluorescent labeling dye, DAPI, on the folding transition of single giant T4 DNA (166 kbp) induced by spermidine3+ were examined, by use of the experimental technique of single molecular chain observation with fluorescence microscopy. It is found that the intercalating dye, YOYO-1, markedly prevents the folding transition, whereas the minor-groove binding dye, DAPI, exhibits negligible effect on the folding transition. This action of YOYO-1 is discussed in relation to the biological effect of intercalators.

Original languageEnglish
Pages (from-to)264-267
Number of pages4
JournalBiochemical and biophysical research communications
Volume286
Issue number2
DOIs
Publication statusPublished - 2001 Jan 1
Externally publishedYes

Keywords

  • DNA
  • Fluorescence microscopy
  • Folding transition
  • Intercalating
  • Minor-groove binding

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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