@article{935737cee08c414bba6fc5ee256ee7bb,
title = "Interaction of N-acetylglutamate kinase with a PII-like protein in rice",
abstract = "PII protein in bacteria is a sensor for 2-oxoglutarate and a transmitter for glutamine signaling. We identified an OsGlnB gene that encoded a bacterial PII-like protein in rice. Yeast two-hybrid analysis showed that an OsGlnB gene product interacted with N-acetylglutamate kinase 1 (OsNAGK1) and PII-like protein (OsGlnB) itself in rice. In cyanobacteria, NAGK is a key enzyme in arginine biosynthesis. Transient expression of OsGlnB cDNA or OsNAGK1 cDNA fused with sGFP in rice leaf blades strongly suggested that the PII-like protein as well as OsNAGK1 protein is located in chloroplasts. Both OsGlnB and OsNAGK1 genes were expressed in roots, leaf blades, leaf sheaths and spikelets of rice, and these two genes were coordinately expressed in leaf blades during the life span. Thus, PII-like protein in rice plants is potentially able to interact with OsNAGK1 protein in vivo. This finding will provide a clue to the precise physiological function of PII-like protein in rice.",
keywords = "N-acetylglutamate kinase, PII-like protein, Rice, Yeast two-hybrid analysis",
author = "Kenjiro Sugiyama and Toshihiko Hayakawa and Toru Kudo and Takashi Ito and Tomoyuki Yamaya",
note = "Funding Information: We thank Dr. T. Nakagawa of Shimane University for providing the binary vector, pGWB5, and the Rice Genome Project of the National Institute of Agrobiological Sciences (NIAS) and the Rice Genome Resource Center (RGRC), Japan, for providing a rice full-length cDNA clone (accession number AK104439). This work was supported in part by a program of CREST of JST (Japan Science Technology), in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 14360035) and in part by The Project for Rice Genome Research (IP6001) from the Ministry of Agriculture, Forestry and Fisheries of Japan. Funding Information: The partial cDNA clone encoding OsNAGK1 was isolated by the yeast two-hybrid screening as described below and the entire OsNAGK1 cDNA clone (accession number AK104439) was provided from the Rice Genome Project of the National Institute of Agrobiological Sciences (NIAS) and the Rice Genome Resource Center (RGRC) (Tsukuba, Ibaraki, Japan). The predicted coding sequence for OsNAGK2 (BAC accession number AP005289, location bp 24,627– 25,921, chromosome 2) was found by database searching in the rice genome sequence using the deduced amino acid sequence for OsNAGK1 cDNA. The cDNA encoding the entire ORF for OsNAGK2 was amplified from shoots of rice seedlings by RT-PCR. The first-strand cDNA was prepared from the total RNA by using a SuperScript First-Strand Synthesis System for RT-PCR kit (Invitrogen, Carlsbad, CA, U.S.A.), as described previously (Suenaga et al. 2003). The 1,130 bp cDNA fragment was obtained from rice shoot cDNA as a template by PCR using KOD-plus DNA polymerase (Toyobo, Tokyo, Japan) with gene-specific primers for OsNAGK2 (the forward primer, 5′-CGTCGAACACCTAGAGCGCA-3′ and the reverse primer, 5′-GCACCAAGATTGATTGTGCACTCAC-3′). This blunt-ended cDNA was cloned at the HincII site of pUC118 vector (Takara) and sequenced.",
year = "2004",
month = dec,
doi = "10.1093/pcp/pch199",
language = "English",
volume = "45",
pages = "1768--1778",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "12",
}