Interaction of N-acetylglutamate kinase with a PII-like protein in rice

Kenjiro Sugiyama, Toshihiko Hayakawa, Toru Kudo, Takashi Ito, Tomoyuki Yamaya

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

PII protein in bacteria is a sensor for 2-oxoglutarate and a transmitter for glutamine signaling. We identified an OsGlnB gene that encoded a bacterial PII-like protein in rice. Yeast two-hybrid analysis showed that an OsGlnB gene product interacted with N-acetylglutamate kinase 1 (OsNAGK1) and PII-like protein (OsGlnB) itself in rice. In cyanobacteria, NAGK is a key enzyme in arginine biosynthesis. Transient expression of OsGlnB cDNA or OsNAGK1 cDNA fused with sGFP in rice leaf blades strongly suggested that the PII-like protein as well as OsNAGK1 protein is located in chloroplasts. Both OsGlnB and OsNAGK1 genes were expressed in roots, leaf blades, leaf sheaths and spikelets of rice, and these two genes were coordinately expressed in leaf blades during the life span. Thus, PII-like protein in rice plants is potentially able to interact with OsNAGK1 protein in vivo. This finding will provide a clue to the precise physiological function of PII-like protein in rice.

Original languageEnglish
Pages (from-to)1768-1778
Number of pages11
JournalPlant and Cell Physiology
Volume45
Issue number12
DOIs
Publication statusPublished - 2004 Dec

Keywords

  • N-acetylglutamate kinase
  • PII-like protein
  • Rice
  • Yeast two-hybrid analysis

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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