Interaction between Theileria orientalis 23-kDa piroplasm membrane protein and heparin

Hitoshi Takemae, Tatsuki Sugi, Kyousuke Kobayashi, Fumi Murakoshi, Frances C. Recuenco, Akiko Ishiwa, Atsuko Inomata, Taisuke Horimoto, Naoaki Yokoyama, Kentaro Kato

Research output: Contribution to journalComment/debate

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Abstract

The 23-kDa piroplasm membrane protein of Theileria orientalis (p23) is an immunogenic protein expressed during the intraerythrocytic stage of the parasite; its function, however, remains unclear. To evaluate the host factor or factors that interact with p23, we examined the binding of p23 to components of the host cell surface. Recombinant p23 protein of the Ikeda genotype failed to bind to bovine red blood cells or to peripheral blood mononuclear cells, but did bind to Madin-Darby Bovine Kidney (MDBK) cells. A glycoarray assay showed that recombinant p23 proteins from the three genotypes bound to heparin, indicating that p23 is a heparin-binding Theileria surface molecule. Further analysis of heparin-binding molecules is useful for understanding attachment and invasion of T. orientalis merozoites.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalJapanese Journal of Veterinary Research
Volume62
Issue number1-2
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Keywords

  • 23-kDa piroplasm membrane protein
  • Glycoarray
  • Heparin

ASJC Scopus subject areas

  • veterinary(all)

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    Takemae, H., Sugi, T., Kobayashi, K., Murakoshi, F., Recuenco, F. C., Ishiwa, A., Inomata, A., Horimoto, T., Yokoyama, N., & Kato, K. (2014). Interaction between Theileria orientalis 23-kDa piroplasm membrane protein and heparin. Japanese Journal of Veterinary Research, 62(1-2), 17-24.