Interaction between leptospiral lipopolysaccharide and toll-like receptor 2 in pig fibroblast cell line, and inhibitory effect of antibody against leptospiral lipopolysaccharide on interaction

Yijie Guo, Tomokazu Fukuda, Shuichi Nakamura, Lanlan Bai, Jun Xu, Kengo Kuroda, Rintaro Tomioka, Hiroshi Yoneyama, Emiko Isogai

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs-NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs-SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalAsian-Australasian Journal of Animal Sciences
Volume28
Issue number2
DOIs
Publication statusPublished - 2015 Feb 1

Keywords

  • Antibody against leptospiral lipopolysaccharide
  • Cytokines
  • Leptospiral lipopolysaccharide
  • Pig fibroblast cell line
  • Toll-like receptor 2

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Engineering(all)

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