Interaction between cells and poly(ethylene glycol)-lipid conjugates

Toru Itagaki; Yusuke Arima; Rei Kuwabara; Narufumi Kitamura; Hiroo Iwata

doi:10.1016/j.colsurfb.2015.08.014

Interaction between cells and poly(ethylene glycol)-lipid conjugates

Toru Itagaki, Yusuke Arima, Rei Kuwabara, Narufumi Kitamura, Hiroo Iwata

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Eight types of poly(ethylene glycol)-lipid(PEG-lipids) carrying different lipid tails were synthesized. These PEG-lipids were labeled with fluorescein isothiocyanate (FITC-PEG-lipids) to examine their interaction with cells and to quantitatively determine amounts of PEG-lipids bound on the cell surface. FITC-PEG-lipids spontaneously anchored to the cell membrane within 15min without loss of cell viability. The type of lipid had very little effect on the anchoring rates, while an increase in the hydrophobicity of the lipid portion of the PEG-lipids slowed their dissociation rates. Densities of FITC-PEG-lipids on the cell surface ranged from 1×10^-3 to 1×10^-2molecules/nm², depending on the kinds of lipids employed. The relationship between the stability of the lipids on the cell membrane and the hydrophobicity of the lipid moieties will give a basis for the selection of a hydrophobic moiety in PEG-lipid conjugates for use in specific applications.

Original language	English
Pages (from-to)	765-773
Number of pages	9
Journal	Colloids and Surfaces B: Biointerfaces
Volume	135
DOIs	https://doi.org/10.1016/j.colsurfb.2015.08.014
Publication status	Published - 2015 Nov 1
Externally published	Yes

Keywords

Amphiphilic polymer
Cell surface modification
Hydrophobic interaction
Poly(ethylene glycol)-lipid conjugates

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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title = "Interaction between cells and poly(ethylene glycol)-lipid conjugates",

abstract = "Eight types of poly(ethylene glycol)-lipid(PEG-lipids) carrying different lipid tails were synthesized. These PEG-lipids were labeled with fluorescein isothiocyanate (FITC-PEG-lipids) to examine their interaction with cells and to quantitatively determine amounts of PEG-lipids bound on the cell surface. FITC-PEG-lipids spontaneously anchored to the cell membrane within 15min without loss of cell viability. The type of lipid had very little effect on the anchoring rates, while an increase in the hydrophobicity of the lipid portion of the PEG-lipids slowed their dissociation rates. Densities of FITC-PEG-lipids on the cell surface ranged from 1×10-3 to 1×10-2molecules/nm2, depending on the kinds of lipids employed. The relationship between the stability of the lipids on the cell membrane and the hydrophobicity of the lipid moieties will give a basis for the selection of a hydrophobic moiety in PEG-lipid conjugates for use in specific applications.",

keywords = "Amphiphilic polymer, Cell surface modification, Hydrophobic interaction, Poly(ethylene glycol)-lipid conjugates",

author = "Toru Itagaki and Yusuke Arima and Rei Kuwabara and Narufumi Kitamura and Hiroo Iwata",

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AU - Itagaki, Toru

AU - Arima, Yusuke

AU - Kuwabara, Rei

AU - Kitamura, Narufumi

AU - Iwata, Hiroo

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Eight types of poly(ethylene glycol)-lipid(PEG-lipids) carrying different lipid tails were synthesized. These PEG-lipids were labeled with fluorescein isothiocyanate (FITC-PEG-lipids) to examine their interaction with cells and to quantitatively determine amounts of PEG-lipids bound on the cell surface. FITC-PEG-lipids spontaneously anchored to the cell membrane within 15min without loss of cell viability. The type of lipid had very little effect on the anchoring rates, while an increase in the hydrophobicity of the lipid portion of the PEG-lipids slowed their dissociation rates. Densities of FITC-PEG-lipids on the cell surface ranged from 1×10-3 to 1×10-2molecules/nm2, depending on the kinds of lipids employed. The relationship between the stability of the lipids on the cell membrane and the hydrophobicity of the lipid moieties will give a basis for the selection of a hydrophobic moiety in PEG-lipid conjugates for use in specific applications.

AB - Eight types of poly(ethylene glycol)-lipid(PEG-lipids) carrying different lipid tails were synthesized. These PEG-lipids were labeled with fluorescein isothiocyanate (FITC-PEG-lipids) to examine their interaction with cells and to quantitatively determine amounts of PEG-lipids bound on the cell surface. FITC-PEG-lipids spontaneously anchored to the cell membrane within 15min without loss of cell viability. The type of lipid had very little effect on the anchoring rates, while an increase in the hydrophobicity of the lipid portion of the PEG-lipids slowed their dissociation rates. Densities of FITC-PEG-lipids on the cell surface ranged from 1×10-3 to 1×10-2molecules/nm2, depending on the kinds of lipids employed. The relationship between the stability of the lipids on the cell membrane and the hydrophobicity of the lipid moieties will give a basis for the selection of a hydrophobic moiety in PEG-lipid conjugates for use in specific applications.

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