TY - JOUR
T1 - Interaction between cells and poly(ethylene glycol)-lipid conjugates
AU - Itagaki, Toru
AU - Arima, Yusuke
AU - Kuwabara, Rei
AU - Kitamura, Narufumi
AU - Iwata, Hiroo
N1 - Funding Information:
This work was financially supported by a Grant-in-Aid for Scientific Research on Innovative Areas “Nanomedicine Molecular Science” (No. 2306 ) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan .
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - Eight types of poly(ethylene glycol)-lipid(PEG-lipids) carrying different lipid tails were synthesized. These PEG-lipids were labeled with fluorescein isothiocyanate (FITC-PEG-lipids) to examine their interaction with cells and to quantitatively determine amounts of PEG-lipids bound on the cell surface. FITC-PEG-lipids spontaneously anchored to the cell membrane within 15min without loss of cell viability. The type of lipid had very little effect on the anchoring rates, while an increase in the hydrophobicity of the lipid portion of the PEG-lipids slowed their dissociation rates. Densities of FITC-PEG-lipids on the cell surface ranged from 1×10-3 to 1×10-2molecules/nm2, depending on the kinds of lipids employed. The relationship between the stability of the lipids on the cell membrane and the hydrophobicity of the lipid moieties will give a basis for the selection of a hydrophobic moiety in PEG-lipid conjugates for use in specific applications.
AB - Eight types of poly(ethylene glycol)-lipid(PEG-lipids) carrying different lipid tails were synthesized. These PEG-lipids were labeled with fluorescein isothiocyanate (FITC-PEG-lipids) to examine their interaction with cells and to quantitatively determine amounts of PEG-lipids bound on the cell surface. FITC-PEG-lipids spontaneously anchored to the cell membrane within 15min without loss of cell viability. The type of lipid had very little effect on the anchoring rates, while an increase in the hydrophobicity of the lipid portion of the PEG-lipids slowed their dissociation rates. Densities of FITC-PEG-lipids on the cell surface ranged from 1×10-3 to 1×10-2molecules/nm2, depending on the kinds of lipids employed. The relationship between the stability of the lipids on the cell membrane and the hydrophobicity of the lipid moieties will give a basis for the selection of a hydrophobic moiety in PEG-lipid conjugates for use in specific applications.
KW - Amphiphilic polymer
KW - Cell surface modification
KW - Hydrophobic interaction
KW - Poly(ethylene glycol)-lipid conjugates
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U2 - 10.1016/j.colsurfb.2015.08.014
DO - 10.1016/j.colsurfb.2015.08.014
M3 - Article
C2 - 26342322
AN - SCOPUS:84940839836
VL - 135
SP - 765
EP - 773
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -