Integrin-linked kinase (ILK) regulation of the cell viability in PTEN mutant glioblastoma and in vitro inhibition by the specific COX-2 inhibitor NS-398

Soichi Obara, Masanori Nakata, Hideo Takeshima, Hideki Katagiri, Tomoichiro Asano, Yoshitomo Oka, Ikuro Maruyama, Jun Ichi Kuratsu

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

We report the increased activity and expression of the ILK protein in human glioblastomas and demonstrate that ILK activity is regulated by PTEN. The transfection of wild type-PTEN into the glioblastoma cell line U-251 MG altered the localization of ILK in the cell membrane; transfection with PTEN down-regulated PKB/Akt-Ser-473 phosphorylation via the inhibition of ILK-signaling. Our results suggest that ILK is critical for the PTEN-sensitive regulation of PKB/Akt-dependent cell survival. The selective COX-2 inhibitor NS-398 was found capable of down-regulating ILK and PKB/Akt phosphorylation. Our data indicate that inhibition of ILK signaling may be beneficial in the treatment of PTEN-deficient glioblastoma.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalCancer Letters
Volume208
Issue number1
DOIs
Publication statusPublished - 2004 May 10
Externally publishedYes

Keywords

  • Glioblastoma
  • ILK
  • NS-398
  • PKB/Akt-Serine 473
  • PTEN

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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