Integration of antibody arrays into a preassembled microfluidic device for on-demand immunoassay

Masahiko Hashimoto, Hirokazu Kaji, Maria E. Kemppinen, Matsuhiko Nishizawa

Research output: Contribution to conferencePaper

Abstract

This paper describes a method to create a patterned surface within a microchannel by electrochemical means, which enables on-demand and site-specific immobilization of protein array after the device is fully assembled. High density protein immobilization is achieved by locating proteins to a three-dimensional microstructure. In addition, by first depositing a pattern of poly(ethylene glycol) (PEG)-based copolymer in the microchannel, a uniform protein pattern is obtained even in a stream flow.

Original languageEnglish
Pages846-848
Number of pages3
Publication statusPublished - 2006 Jan 1
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 2006 Nov 52006 Nov 9

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period06/11/506/11/9

Keywords

  • 3D microstructures
  • Electrochemical method
  • Microchannel
  • Protein pattern

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Hashimoto, M., Kaji, H., Kemppinen, M. E., & Nishizawa, M. (2006). Integration of antibody arrays into a preassembled microfluidic device for on-demand immunoassay. 846-848. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.