A volatile chemical, dibromomethane (DBM), produced from red coralline algae is known as a chemical inducer of larval metamorphosis of the sea urchin Strongylocentrotus nudus. We performed experiments exposing DBM to the larvae of S. nudus and Strongylocentrotus intermedius through a hydrophobic membrane. Metamorphic rates resulting from different diluted DBM solutions and exposure times were ascertained. The highest metamorphic rate, more than 80% in both species, was found after 1 h exposure to 1/2 diluted DBM. With this dilution, more than 80% of S. nudus and S. intermedius larvae metamorphosed 1 h after start of the experiment after only 10 and 5 min exposure, respectively, which corresponded to the low concentrations of 52-61 ppm and 34-43 ppm DBM by GCMS analysis, respectively. These findings suggest that DBM has an instantaneous effect on high success of metamorphosis of larvae of S. nudus and S. intermedius.
- Sea urchin
- Strongylocentrotus intermedius
- Strongylocentrotus nudus
ASJC Scopus subject areas
- Aquatic Science