Insect pheromone behavior: Fruit fly

Daisuke Yamamoto, Soh Kohatsu, Masayuki Koganezawa

    Research output: Chapter in Book/Report/Conference proceedingChapter

    1 Citation (Scopus)


    The amenability to genetics of Drosophila melanogaster has made this organism one of the best-suited models for studying the neurobiology of pheromone-guided behavior. Single-male assays use the minigene encoding the thermosensitive channel dTrpA1 to activate neurons expressing fruitless (fru), a major courtship regulator gene, and thereby induce most of the elementary courtship acts in a solitary male exposed to temperature increase. Tethered male assays allow Ca2+-imaging of neuronal activities of a male fly displaying courtship behavior on a treadmill when stimulated with a female or pheromones. Here we describe technical details of these assays.

    Original languageEnglish
    Title of host publicationPheromone Signaling
    Subtitle of host publicationMethods and Protocols
    EditorsKazushige Touhara
    Number of pages12
    Publication statusPublished - 2013 Sep 20

    Publication series

    NameMethods in Molecular Biology
    ISSN (Print)1064-3745


    • Ca-imaging
    • Courtship
    • Drosophila
    • MARCM
    • Thermogenetics
    • fruitless

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics


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