Insect pheromone behavior: Fruit fly

Daisuke Yamamoto, Soh Kohatsu, Masayuki Koganezawa

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    The amenability to genetics of Drosophila melanogaster has made this organism one of the best-suited models for studying the neurobiology of pheromone-guided behavior. Single-male assays use the minigene encoding the thermosensitive channel dTrpA1 to activate neurons expressing fruitless (fru), a major courtship regulator gene, and thereby induce most of the elementary courtship acts in a solitary male exposed to temperature increase. Tethered male assays allow Ca2+-imaging of neuronal activities of a male fly displaying courtship behavior on a treadmill when stimulated with a female or pheromones. Here we describe technical details of these assays.

    Original languageEnglish
    Title of host publicationPheromone Signaling
    Subtitle of host publicationMethods and Protocols
    EditorsKazushige Touhara
    Pages261-272
    Number of pages12
    DOIs
    Publication statusPublished - 2013 Sep 20

    Publication series

    NameMethods in Molecular Biology
    Volume1068
    ISSN (Print)1064-3745

    Keywords

    • Ca-imaging
    • Courtship
    • Drosophila
    • MARCM
    • Thermogenetics
    • fruitless

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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