@inbook{c39d88cec2ed41dc9d24300ab5417f9c,
title = "Insect pheromone behavior: Fruit fly",
abstract = "The amenability to genetics of Drosophila melanogaster has made this organism one of the best-suited models for studying the neurobiology of pheromone-guided behavior. Single-male assays use the minigene encoding the thermosensitive channel dTrpA1 to activate neurons expressing fruitless (fru), a major courtship regulator gene, and thereby induce most of the elementary courtship acts in a solitary male exposed to temperature increase. Tethered male assays allow Ca2+-imaging of neuronal activities of a male fly displaying courtship behavior on a treadmill when stimulated with a female or pheromones. Here we describe technical details of these assays.",
keywords = "Ca-imaging, Courtship, Drosophila, MARCM, Thermogenetics, fruitless",
author = "Daisuke Yamamoto and Soh Kohatsu and Masayuki Koganezawa",
year = "2013",
month = sep,
day = "20",
doi = "10.1007/978-1-62703-619-1_19",
language = "English",
isbn = "9781627036184",
series = "Methods in Molecular Biology",
pages = "261--272",
editor = "Kazushige Touhara",
booktitle = "Pheromone Signaling",
}