Injection of sperm extract mimics spatiotemporal dynamics of Ca2+ responses and progression of meiosis at fertilization of ascidian oocytes

Keiichiro Kyozuka, Ryusaku Deguchi, Tatsuma Mohrl, Shunichi Miyazaki

Research output: Contribution to journalArticlepeer-review

83 Citations (Scopus)

Abstract

Sperm extract (SE) of the ascidian, Ciona savignyi, injected into oocytes induced repetitive intracellular Ca2+ increases with kinetics consistent with those at fertilization and caused reinitiation and progression of meiosis as in fertilized oocytes with the formation of polar bodies. The Ca2+ response comprised two sets of Ca2+ oscillations separated by 5 minutes and correlated with the first and second meiotic metaphase. The effects of SE were dose dependent and the critical dose corresponded roughly to a single spermatozoon. In the first Ca2+ transient observed by confocal microscopy, a Ca2+ wave started from the SE injection site at the peripheral region of the oocyte and propagated across the ooplasm. The similar wave was produced by injection at the central region, starting from an arbitrary cortical area after 30 seconds, probably after SE had diffused to the cortex. The sensitivity to SE is thought to be preferentially higher in the cortex. The effective component of SE was heat-unstable, and its molecular weight was estimated as in the range between 10 x 104 and 3 x 104 using membrane filters. These results suggest that, in ascidian fertilization, a cytosolic sperm protein factor is introduced to the oocyte cortex and induces Ca2+ waves and thereby meiotic resumption, leading to cell-cycle-correlated Ca2+ oscillations.

Original languageEnglish
Pages (from-to)4099-4105
Number of pages7
JournalDevelopment
Volume125
Issue number20
Publication statusPublished - 1998 Nov 23

Keywords

  • Ascidian oocyte
  • Calcium oscillation
  • Calcium wave
  • Fertilization
  • Intracellular calcium
  • Meiotic division
  • Sperm factor

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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