Inhibitory interaction of c-Myb and GATA-1 via transcriptional co-activator CBP

Tomomi Takahashi, Naruyoshi Suwabe, Ping Dai, Masayuki Yamamoto, Shunsuke Ishii, Toru Nakano

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Gene targeting experiments have revealed that transcription factors such as c-Myb and GATA-1 play crucial roles during hematopoietic differentiation. c-Myb is necessary in the immature cells of almost every hematopoietic lineage and GATA-1 is essential for the development of the erythroid lineage. In addition, CREB-binding protein (CBP) acts as a transcriptional adapter for various transcription factors, including c-Myb and GATA-1. In this paper, we show that the transcription factors c-Myb and GATA-1 each inhibit the transcriptional activity of the other and that any possible bipartite complexes c-Myb, GATA-1, and CBP could be formed, but the tripartite complex was hardly formed. The exclusive binding of GATA-1 and c-Myb to CBP is probably the molecular basis for the mutual inhibition of their transcriptional activity. Our data suggest that cross-talk between these three factors might be important for hematopoietic differentiation and that CBP functions as a key molecule during the process.

Original languageEnglish
Pages (from-to)134-140
Number of pages7
JournalOncogene
Volume19
Issue number1
DOIs
Publication statusPublished - 2000 Jan 6
Externally publishedYes

Keywords

  • CBP
  • Cell differentiation
  • GATA-1
  • Hematopoiesis
  • c-Myb

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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