Inhibitory effects of prostaglandin a2 on c-myc expression and cell cycle progression in human leukemia cell line hl-60

Chikashi Ishioka, Ryunosuke Kanamaru, Toshiaki Sato, Toshio Dei, Yukari Konishi, Mitsuo Asamura, Akira Wakui

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Abstract

The effect of prostaglandin A2 (PGA2) on c-myc expression was investigated in a human promyelocytic leukemia cell line, HL-60, which responded to PGA2 with a dose-dependent growth inhibition. Northern blot analysis indicated that treatment with PGA2 at 0.5 to 5.0 μg/ml remarkably reduced the steady state level of c-myc mRNA within 3 h, and then it gradually recovered according to the order of concentration of the drug. In contrast to c-myc, the level of class I HLA mRNA, as an internal control, was not diminished by PGA2 treatment. Further, this reduction of c-myc was not disturbed by cycloheximide, suggesting that this PGA2 action on c-myc expression is independent of de novo protein synthesis. Cytofluorometric analysis revealed that the exposure of HL-60 cells to PGA2 at 0.5 or 5.0 μg/ml arrested the cells in the G0-G1 phase of the cell cycle. This accumulation of the cells in G0-G1 phase continues until 24 or 36 h at 0.5 or 5.0 μg/ml, respectively. The G0-G1 arrest of the cell cycle was also recovered as the inhibition of c-myc was released. This recovery may be due to the loss of activity of PGA2 in culture medium. This study clearly showed that PGA2 treatment arrested HL-60 cells in the G0-G1 phase of the cell cycle and was associated with the reduction of c-myc mRNA.

Original languageEnglish
Pages (from-to)2813-2818
Number of pages6
JournalCancer Research
Volume48
Issue number10
Publication statusPublished - 1988 May

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Ishioka, C., Kanamaru, R., Sato, T., Dei, T., Konishi, Y., Asamura, M., & Wakui, A. (1988). Inhibitory effects of prostaglandin a2 on c-myc expression and cell cycle progression in human leukemia cell line hl-60. Cancer Research, 48(10), 2813-2818.