Inhibitory effects of PLAP-1/asporin on periodontal ligament cells

T. Kajikawa, S. Yamada, T. Tauchi, T. Awata, S. Yamaba, C. Fujihara, S. Murakami

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

PLAP-1/asporin is an extracellular matrix protein that is predominantly expressed in the human periodontal ligament (PDL) and has an aspartic acid (D) repeat polymorphism in its N-terminal region. In this study, we hypothesized that the D repeat polymorphism of PLAP-1/asporin may affect the physiological functions of periodontal ligaments. We established periodontal ligament cell lines transfected with the D13- or D14-PLAP-1 gene. Alkaline phosphatase staining and alizarin red staining revealed that the cytodifferentiation of the D14-PLAP-1-expressing PDL cells was more repressed compared with that of the D13-PLAP-1-expressing cells. Furthermore, the D14-PLAP-1-expressing cells inhibited BMP-2-induced cytodifferentiation more strongly than did the D13-PLAP-1-expressing cells. Western blotting analysis and luciferase assay revealed that D14-PLAP-1 suppressed BMP-2 signal transduction more efficiently than did D13-PLAP-1, and co-immunoprecipitation demonstrated the stronger affinity of the D14-PLAP-1 protein to BMP-2 compared with the D13-PLAP-1 protein. Analysis of these data suggests that the D repeat polymorphism of PLAP-1/asporin has a significant influence on the functions of PDL cells.

Original languageEnglish
Pages (from-to)400-405
Number of pages6
JournalJournal of dental research
Volume93
Issue number4
DOIs
Publication statusPublished - 2014 Apr
Externally publishedYes

Keywords

  • BMP-2
  • asporin
  • cytodifferentiation
  • extracellular matrix
  • gene polymorphism
  • tissue homeostasis

ASJC Scopus subject areas

  • Dentistry(all)

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