Inhibitory effects of ovoglobulins on bacillary necrosis in larvae of the Pacific oyster, Crassostrea gigas

Keisuke G. Takahashi, Akifumi Nakamura, Katsuyoshi Mori

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

In order to develop an alternative method to antibiotics for preventing bacillary necrosis in bivalve mollusc larvae, we examined the effects of ovoglobulins (proteins derived from the whites of hens' eggs) on the survival of larvae of the Pacific oyster Crassostrea gigas. The pathogenic Vibrio tubiashii (ATCC 19106) was used to infect larvae of the Pacific oyster. V. tubiashii showed strong pathogenicity to oyster larvae, causing 100% mortality after experimental exposure for 24 h at a concentration of 105 cfu (colony-forming units)/ml. In contrast, the addition of ovoglobulins at a concentration of 10 μg/ml to larval oysters, challenged with V. tubiashii at 105 cfu/ml, led to a marked increase in larval survival of 96.5% at 24 h after infection. The V. tubiashii culture supernatant was also shown to be pathogenic to larval oysters; however, its pathogenicity was completely inhibited by the addition of 10 μg/ml of ovoglobulins. Larval oysters infected by V. tubiashii showed typical symptoms of baciliary necrosis including anomalous swimming and detachment of cilia and/or vela. In contrast, live larvae were actively motile, and their cilia and vela were not necrotized in the ovoglobulins-added group. The addition of ovoglobulins clearly suppressed the growth of V. tubiashii in gelatin-sea water broth, but the number of viable V. tubiashii 24 h after incubation did not decrease to the initial dose level. Findings obtained in this study indicate that ovoglobulins almost completely protect larval oysters from V. tubiashii infection by nonbactericidally inhibiting the growth of V. tubiashii without affecting survival of the oysters. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)212-217
Number of pages6
JournalJournal of Invertebrate Pathology
Volume75
Issue number3
DOIs
Publication statusPublished - 2000 Apr

Keywords

  • Bacillary necrosis
  • Bivalve molluscs
  • Crassostrea gigas
  • Larvae
  • Ovoglobulins
  • Vibrio tubiashii

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

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