Inhibitory effects of nitrite on acid production in dental plaque in children

Yuji Yamamoto, Jumpei Washio, Koichi Shimizu, Koei Igarashi, Nobuhiro Takahashi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Purpose: To assess the inhibitory effects of nitrite on plaque acidogenicity and its relationship with caries experience. Materials and Methods: Plaque (2 μl) was collected from 76 children (age 5.8 ± 2.6 years, dmft 2.9 ± 3.5, DMTF 0.6 ± 1.4) and mixed with nitrite solution (final concentration = 0.63 mM) or distilled water (control). The initial pH (pH-0) of each sample was measured using a portable pH meter. The samples were incubated for 10 min, then their pH (pH-1) was measured again. Next, glucose (final concentration = 0.67%) was added to the samples, which were then incubated for a further 10 min before their pH was assessed for a third time (pH-2). Results: The pH-0, pH-1, and pH-2 values of the control samples were 7.25 ± 0.16, 6.07 ± 0.44, and 5.11 ± 0.48, respectively, and those of the nitrite-treated samples were 7.26 ± 0.16, 6.37 ± 0.45, and 5.34 ± 0.48, respectively. The pH-1 and pH-2 values of the nitrite-treated samples were higher than those of the control samples (p < 0.005). Greater plaque acid production was associated with stronger inhibition of plaque acid production by nitrite (p < 0.005). No relationship was detected between the inhibition by nitrite and caries experience. Conclusions: Nitrite inhibited both endogenous and exogenous plaque acid production. Nitrite inhibited acid production more markedly in plaque that exhibited greater acid production, suggesting that nitrite might be effective at preventing caries, as it contributes to pH homeostasis in plaque by countering excess acidification.

Original languageEnglish
Pages (from-to)153-156
Number of pages4
JournalOral Health and Preventive Dentistry
Volume15
Issue number2
DOIs
Publication statusPublished - 2017

Keywords

  • Acid production
  • Dental plaque
  • Nitrate
  • Nitrite
  • PH

ASJC Scopus subject areas

  • Dental Hygiene

Fingerprint Dive into the research topics of 'Inhibitory effects of nitrite on acid production in dental plaque in children'. Together they form a unique fingerprint.

Cite this