Inhibitory effects of nicotine derived from cigarette smoke on thymic stromal lymphopoietin production in epidermal keratinocytes

Jiangxu Dong, Ryosuke Segawa, Natsumi Mizuno, Masahiro Hiratsuka, Noriyasu Hirasawa

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Thymic stromal lymphopoietin (TSLP) is regarded as the main factor responsible for the pathogenesis of atopic dermatitis (AD). Cigarette smoke is an aggravating factor for allergies, but has been reported to decrease the risk of AD. In the present study, we evaluated the role of nicotine, the main constituent in cigarette smoke extract, and its underlying mechanism of action in the regulation of TSLP expression. We found that nicotine significantly inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced TSLP expression in BALB/c mice and the mouse keratinocyte cell line PAM212. Nicotine inhibition of TSLP production was abolished by pretreatments with α7 nicotinic acetylcholine receptor (α7 nAChR) antagonists, AMP-activated protein kinase (AMPK) inhibitor, and phosphoinositide 3-kinase (PI3K) inhibitors. The same inhibitors abolished inhibition of nuclear factor-κB (NF-κB) activation by nicotine. These results suggest that nicotine inhibits the expression of TSLP by suppressing the activation of NF-κB through the α7 nAChR-PI3K-AMPK signaling pathway.

Original languageEnglish
Pages (from-to)19-25
Number of pages7
JournalCellular Immunology
Volume302
DOIs
Publication statusPublished - 2016 Apr 1

Keywords

  • AD
  • CSE
  • Cell signaling
  • Nicotine
  • TSLP

ASJC Scopus subject areas

  • Immunology

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