Inhibitory effects of hot water extract of the stevia stem on the contractile response of the smooth muscle of the guinea pig ileum

Kazuhiro Shiozaki, Akiko Fujii, Toshiki Nakano, Toshiyasu Yamaguchi, Minoru Sato

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The effects of a hot water extract of the stem of Stevia rebaudiana on the smooth muscle of isolated guinea pig ileum were investigated. The butyl alcohol layer of the extract antagonized the contractions of the isolated guinea pig ileum induced by histamine (1 × 10-5 M) and acetylcholine (1 × 10-5 M) in a concentration-dependent manner. The butyl alcohol layer of the extract also showed inhibition of CaCl2 (1 × 10-3-3.8 × 10-1 M)-induced contractions. The antagonism of the extract was considered to be non-specific, but this action might be related to an influx of extracellular Ca2+. With column chromatography preparation, the active component was assumed to be as stevioside. The antagonistic effects exerted by the stem extract of Stevia rebaudiana contributed to the gastroprotective activity of the extract in animals fed dietary histamine.

Original languageEnglish
Pages (from-to)489-494
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number2
DOIs
Publication statusPublished - 2006

Keywords

  • Calcium channel blocker
  • Spasmolytic
  • Stevia rebaudiana
  • Stevioside

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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