Inhibitory effect of sorbitol on sugar metabolism of Streptococcus mutans in vitro and on acid production in dental plaque in vivo

S. Takahashi-Abbe, K. Abbe, N. Takahashi, Y. Tamazawa, T. Yamada

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

This study was conducted to find out whether sorbitol inhibits the sugar metabolism of Streptococcus mutans in vitro and the acid production in dental plaque in vivo. S. mutans NCIB 11723 was anaerobically grown in sorbitol-containing medium. The rate of acid production from sugars was estimated with a pH stat. The rate of acid production from glucose or sucrose was not changed at various concentrations of oxygen. By the addition of sorbitol to sugar, however, the acid production was decreased with increasing levels of oxygen. Intracellular NADH/NAD+ ratio and (dihydroxyacetone-phosphate+glyceraldehyde-phosphate)/3-phosphogl ycerate ratio were high whenever the acid production was inhibited by sorbitol. Sorbitol also inhibited the acid production in dental plaque in vivo. These results suggest that the increased NADH/NAD+ ratio during sorbitol metabolism through the inactivation of pyruvate formate-lyase by oxygen inhibited glyceraldehyde-phosphate dehydrogenase and then the acid production of S. mutans and the one in dental plaque.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalOral Microbiology and Immunology
Volume16
Issue number2
DOIs
Publication statusPublished - 2001

Keywords

  • Acid production
  • Dental plaque-pH
  • In-dwelling telemetry
  • Inhibition
  • NADH/NAD ratio
  • Pyruvate formate-lyase
  • Sorbitol
  • Streptococcus mutans
  • Sugar metabolism
  • Sugar-alcohol

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)

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