Inhibitory effect of Pseudomonas putida nitrogen-related phosphotransferase system on conjugative transfer of IncP-9 plasmid from Escherichia coli

Kei Inoue, Ryo Miyazaki, Yoshiyuki Ohtsubo, Yuji Nagata, Masataka Tsuda

    Research output: Contribution to journalLetter

    5 Citations (Scopus)

    Abstract

    Conjugative plasmid transfer systems have been well studied, but very little is known about the recipient factors that control horizontal transmission. A self-transmissible IncP-9 naphthalene catabolic plasmid, NAH7, carries the traF gene, whose product is considered to be a host-range modifier of NAH7, because its traF deletion mutant (NAH7dF) is transmissible from Pseudomonas putida to P. putida and Escherichia coli and from E. coli to E. coli, but not from E. coli to P. putida. In this study, transposon mutagenesis of P. putida KT2440 was performed to isolate the mutants that could receive NAH7dF from E. coli. The mutants had the transposon insertions in ptsP or ptsO, encoding two of three components of the nitrogen-related phosphotransferase system (PTSNtr). The KT2440 derivative lacking ptsN, encoding the remaining component of PTSNtr, was also able to receive NAH7dF. These results indicated that the PTSNtr in P. putida is involved in inhibition of conjugative transfer of NAH7dF from E. coli. Our further experiments using site-directed mutants suggested the indirect involvement of the phosphorylated form of PtsO in the inhibition of the conjugative transfer. Conjugative transfer of NAH7 and another IncP-9 plasmid, pWW0, from E. coli was partially inhibited by the PtsO function in KT2440.

    Original languageEnglish
    Pages (from-to)102-109
    Number of pages8
    JournalFEMS Microbiology Letters
    Volume345
    Issue number2
    DOIs
    Publication statusPublished - 2013 Aug

    Keywords

    • Conjugation
    • Host range
    • IncP-9 plasmid
    • PTS
    • Pseudomonas

    ASJC Scopus subject areas

    • Microbiology
    • Molecular Biology
    • Genetics

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