Inhibitory effect of pranidipine on N-type voltage-dependent Ca 2+ channels in mice

Manabu Murakami, Toyoki Mori, Osamu Nakagawasai, Kunie Hagiwara, Yumiko Sakurada, Izadi I. Mobarakeh, Atsunobu Murata, Fumihiro Yamadera, Ichiro Miyoshi, Koichi Tan-No, Kazuhiko Yanai, Takeshi Tadano, Toshihiko Iijima

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We investigated the N-type voltage-dependent calcium channel blocking action of pranidipine, a novel dihydropyridine (DHP) derivative. Pranidipine significantly suppressed KCl-induced intracellular calcium changes ([Ca 2+]i) in a dose-dependent fashion in dorsal root ganglion neurons. A patch-clamp investigation revealed a dose-dependent blocking effect on N-type currents. Intrathecal injection of pranidipine significantly shortened the licking time in the late phase of the formalin test, as occurs with cilnidipine and amlodipine, which act on L- and N-type channels. Conversely, nicardipine, which acts exclusively on L-type channels, had no antinociceptive effect. Our results indicate that pranidipine inhibits N-type calcium channels. Furthermore, it exerts an antinociceptive effect, which might be related to an attenuation of synaptic transmission by nociceptive neurons due to the blocking effect of pranidipine on N-type calcium channels in primary nociceptive afferent fibers.

Original languageEnglish
Pages (from-to)118-122
Number of pages5
JournalNeuroscience Letters
Volume367
Issue number1
DOIs
Publication statusPublished - 2004 Aug 26

Keywords

  • Calcium channels
  • DRG neurons
  • Dihydropyridine
  • Mice
  • N-type channels

ASJC Scopus subject areas

  • Neuroscience(all)

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