Inhibitory effect of all-trans retinoic acid on androgen-induced growth of mouse seminal vesicles in vivo and its mechanism

Hideki Sato, Nozomu Tanji, Motomu Tsuji, Nobuyuki Terada, Kenshi Yamasaki, Jianbo Wang, Kenshi Sakayama, Masayoshi Yokoyama

Research output: Contribution to journalArticlepeer-review

Abstract

We examined the effect of all-trans retinoic acid (ATRA) on the androgen-induced growth of mouse seminal vesicles (SVs) in vivo and its mechanisms. Testosterone propionate (TP) alone or with ATRA was injected daily into adult castrated BALB/c mice. Injections of ATRA significantly inhibited the TP-induced growth of SVs in terms of wet weight and DNA synthesis by a pair of SVs evaluated by [3H]thymidine uptake. The bromodeoxyuridine labelling index showed that ATRA inhibited the proliferation of both epithelial and stromal cells. Immunoreactivity for retinoic acid receptor-α was found in the basal epithelial cells. Injections of ATRA affected neither 5α-reductase activity nor the expression of mRNAs for TGF-β1, 2 and 3 and TGF-β receptor 1 and 2 in the SVs. However, androgen receptor (AR) binding assays and Western blotting revealed a decrease in AR without a change in ligand-binding affinity. The present study showed that retinoid inhibited the androgen-induced growth of mouse SVs in vivo, and suggests that a decrease in AR is one of its mechanisms.

Original languageEnglish
Pages (from-to)467-474
Number of pages8
JournalHuman and Experimental Toxicology
Volume24
Issue number9
DOIs
Publication statusPublished - 2005 Sep
Externally publishedYes

Keywords

  • Androgen receptor
  • RAR-α
  • Retinoic acid
  • Seminal vesicle
  • in vivo

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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