TY - JOUR
T1 - Inhibitory actions of prostaglandin E1 on neurogenic plasma extravasation in rat airways
AU - Masatoshi Morikawa, Morikawa
AU - Takashi Aikawa, Aikawa
AU - Kiyohisa Sekizawa, Sekizawa
AU - Takashi Ohrui, Ohrui
AU - Hidetada Sasaki, Sasaki
AU - Tamotsu Takishima, Takishima
PY - 1992/6/24
Y1 - 1992/6/24
N2 - To determine whether neurogenic inflammation can be inhibited by prostaglandin E1 (PGE1), that is suggested to have an inhibitory effect on neuropeptide release from airway sensory nerves, we examined plasma extravasation in the airways of anesthetized rats in vivo with Evans blue due as a marker. Neurogenic inflammation was produced by an i.v. injection of capsaicin (100 μg/kg) or by antidromic electrical stimulation of the right vagus nerve (4 Hz, 1 ms, 4 V for 1 min). Capsaicin injection significantly increased leakage of dye in the trachea and main bronchi. Similar increases in leakage were seen in the trachea and right bronchus on electrical stimulation of the right vagus nerve. PGE1 (1-1000 μg/kg) inhibited the leakage induced by capsaicin in the trachea and bronchi concentration dependently with complete inhibition at a concentration of 1000 μg/kg. Likewise, PGE1 (1000 μg/kg) significantly inhibited electrical stimulation-induced leakage in the trachea and right bronchus (P < 0.01). I.v. substance P (SP; 1 μg/kg) increased Evans blue dye extravasation in the same way as the leakage induced by capsaicin and electrical stimulation but PGE1 (1000 μg/kg) failed to inhibit SP-induced leakage in the trachea and main bronchi (P > 0.20). These results suggest that PGE1 inhibits neurogenic plasma leakage by presynaptic inhibition of the release of neuropeptides from sensory nerves.
AB - To determine whether neurogenic inflammation can be inhibited by prostaglandin E1 (PGE1), that is suggested to have an inhibitory effect on neuropeptide release from airway sensory nerves, we examined plasma extravasation in the airways of anesthetized rats in vivo with Evans blue due as a marker. Neurogenic inflammation was produced by an i.v. injection of capsaicin (100 μg/kg) or by antidromic electrical stimulation of the right vagus nerve (4 Hz, 1 ms, 4 V for 1 min). Capsaicin injection significantly increased leakage of dye in the trachea and main bronchi. Similar increases in leakage were seen in the trachea and right bronchus on electrical stimulation of the right vagus nerve. PGE1 (1-1000 μg/kg) inhibited the leakage induced by capsaicin in the trachea and bronchi concentration dependently with complete inhibition at a concentration of 1000 μg/kg. Likewise, PGE1 (1000 μg/kg) significantly inhibited electrical stimulation-induced leakage in the trachea and right bronchus (P < 0.01). I.v. substance P (SP; 1 μg/kg) increased Evans blue dye extravasation in the same way as the leakage induced by capsaicin and electrical stimulation but PGE1 (1000 μg/kg) failed to inhibit SP-induced leakage in the trachea and main bronchi (P > 0.20). These results suggest that PGE1 inhibits neurogenic plasma leakage by presynaptic inhibition of the release of neuropeptides from sensory nerves.
KW - Capsaicin
KW - Neurogenic inflammation
KW - Neuropeptides
UR - http://www.scopus.com/inward/record.url?scp=0026750538&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026750538&partnerID=8YFLogxK
U2 - 10.1016/0014-2999(92)90507-Z
DO - 10.1016/0014-2999(92)90507-Z
M3 - Article
C2 - 1383005
AN - SCOPUS:0026750538
VL - 217
SP - 31
EP - 35
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1
ER -