Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa

Koji Muramoto; Hiroshi Yako; Koji Murakami; Satoshi Odo; Hisao Kamiya

doi:10.1016/0305-0491(94)90204-6

Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa

Koji Muramoto, Hiroshi Yako, Koji Murakami, Satoshi Odo, Hisao Kamiya

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

The interaction between the multiple lectins (BRA-2, BRA-3) in the coelomic fluid of the acorn barnacle Megabalanus rosa and calcium ions was studied by ultraviolet difference spectroscopy. The association constants for BRA-2 and BRA-3 were 1.4 × 10⁴ M^-1 and 2.2 × 10⁴ M^-1 at pH 8.0, respectively. Multiple lectins inhibited the crystal growth of supersaturated calcium carbonate solution at a lectin concentration of > 0.1 mg/30 ml. Destruction of the carbohydrate-recognition domains of the lectins decreased the inhibitory activities on the crystal growth. Incorporation of BRAs into the crystal was demonstrated using FITC-labeled lectins.

Original language	English
Pages (from-to)	401-409
Number of pages	9
Journal	Comparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume	107
Issue number	3
DOIs	https://doi.org/10.1016/0305-0491(94)90204-6
Publication status	Published - 1994 Mar

Keywords

Calcium carbonate
Lectins
Megabalanus rosa
Recognition domains

ASJC Scopus subject areas

Biochemistry
Physiology
Molecular Biology

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10.1016/0305-0491(94)90204-6

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Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa. / Muramoto, Koji; Yako, Hiroshi; Murakami, Koji; Odo, Satoshi; Kamiya, Hisao.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 107, No. 3, 03.1994, p. 401-409.

Research output: Contribution to journal › Article › peer-review

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title = "Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa",

abstract = "The interaction between the multiple lectins (BRA-2, BRA-3) in the coelomic fluid of the acorn barnacle Megabalanus rosa and calcium ions was studied by ultraviolet difference spectroscopy. The association constants for BRA-2 and BRA-3 were 1.4 × 104 M-1 and 2.2 × 104 M-1 at pH 8.0, respectively. Multiple lectins inhibited the crystal growth of supersaturated calcium carbonate solution at a lectin concentration of > 0.1 mg/30 ml. Destruction of the carbohydrate-recognition domains of the lectins decreased the inhibitory activities on the crystal growth. Incorporation of BRAs into the crystal was demonstrated using FITC-labeled lectins.",

keywords = "Calcium carbonate, Lectins, Megabalanus rosa, Recognition domains",

author = "Koji Muramoto and Hiroshi Yako and Koji Murakami and Satoshi Odo and Hisao Kamiya",

note = "Funding Information: Acknowledgements--This work was supportedin part by grantsfr omNew Energya ndI ndustriaTl echnologDy evel-opmenOt rganizationa,n dthe ResearcIhn stituteo f Marine Invertebrateasn, d a Grant-in-Aidfo r ScientificR esearch from the Ministry of EducationS, ciencea nd Cultureo f Japan. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1994",

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doi = "10.1016/0305-0491(94)90204-6",

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AU - Odo, Satoshi

AU - Kamiya, Hisao

N1 - Funding Information: Acknowledgements--This work was supportedin part by grantsfr omNew Energya ndI ndustriaTl echnologDy evel-opmenOt rganizationa,n dthe ResearcIhn stituteo f Marine Invertebrateasn, d a Grant-in-Aidfo r ScientificR esearch from the Ministry of EducationS, ciencea nd Cultureo f Japan. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1994/3

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N2 - The interaction between the multiple lectins (BRA-2, BRA-3) in the coelomic fluid of the acorn barnacle Megabalanus rosa and calcium ions was studied by ultraviolet difference spectroscopy. The association constants for BRA-2 and BRA-3 were 1.4 × 104 M-1 and 2.2 × 104 M-1 at pH 8.0, respectively. Multiple lectins inhibited the crystal growth of supersaturated calcium carbonate solution at a lectin concentration of > 0.1 mg/30 ml. Destruction of the carbohydrate-recognition domains of the lectins decreased the inhibitory activities on the crystal growth. Incorporation of BRAs into the crystal was demonstrated using FITC-labeled lectins.

AB - The interaction between the multiple lectins (BRA-2, BRA-3) in the coelomic fluid of the acorn barnacle Megabalanus rosa and calcium ions was studied by ultraviolet difference spectroscopy. The association constants for BRA-2 and BRA-3 were 1.4 × 104 M-1 and 2.2 × 104 M-1 at pH 8.0, respectively. Multiple lectins inhibited the crystal growth of supersaturated calcium carbonate solution at a lectin concentration of > 0.1 mg/30 ml. Destruction of the carbohydrate-recognition domains of the lectins decreased the inhibitory activities on the crystal growth. Incorporation of BRAs into the crystal was demonstrated using FITC-labeled lectins.

KW - Calcium carbonate

KW - Lectins

KW - Megabalanus rosa

KW - Recognition domains

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Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa

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Keywords

ASJC Scopus subject areas

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