Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa

Koji Muramoto, Hiroshi Yako, Koji Murakami, Satoshi Odo, Hisao Kamiya

    Research output: Contribution to journalArticlepeer-review

    27 Citations (Scopus)

    Abstract

    The interaction between the multiple lectins (BRA-2, BRA-3) in the coelomic fluid of the acorn barnacle Megabalanus rosa and calcium ions was studied by ultraviolet difference spectroscopy. The association constants for BRA-2 and BRA-3 were 1.4 × 104 M-1 and 2.2 × 104 M-1 at pH 8.0, respectively. Multiple lectins inhibited the crystal growth of supersaturated calcium carbonate solution at a lectin concentration of > 0.1 mg/30 ml. Destruction of the carbohydrate-recognition domains of the lectins decreased the inhibitory activities on the crystal growth. Incorporation of BRAs into the crystal was demonstrated using FITC-labeled lectins.

    Original languageEnglish
    Pages (from-to)401-409
    Number of pages9
    JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
    Volume107
    Issue number3
    DOIs
    Publication statusPublished - 1994 Mar

    Keywords

    • Calcium carbonate
    • Lectins
    • Megabalanus rosa
    • Recognition domains

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology
    • Molecular Biology

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