Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa

Koji Muramoto, Hiroshi Yako, Koji Murakami, Satoshi Odo, Hisao Kamiya

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The interaction between the multiple lectins (BRA-2, BRA-3) in the coelomic fluid of the acorn barnacle Megabalanus rosa and calcium ions was studied by ultraviolet difference spectroscopy. The association constants for BRA-2 and BRA-3 were 1.4 × 104 M-1 and 2.2 × 104 M-1 at pH 8.0, respectively. Multiple lectins inhibited the crystal growth of supersaturated calcium carbonate solution at a lectin concentration of > 0.1 mg/30 ml. Destruction of the carbohydrate-recognition domains of the lectins decreased the inhibitory activities on the crystal growth. Incorporation of BRAs into the crystal was demonstrated using FITC-labeled lectins.

Original languageEnglish
Pages (from-to)401-409
Number of pages9
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume107
Issue number3
DOIs
Publication statusPublished - 1994 Mar
Externally publishedYes

Keywords

  • Calcium carbonate
  • Lectins
  • Megabalanus rosa
  • Recognition domains

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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