Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa

Koji Muramoto; Hiroshi Yako; Koji Murakami; Satoshi Odo; Hisao Kamiya

doi:10.1016/0305-0491(94)90204-6

Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa

Koji Muramoto, Hiroshi Yako, Koji Murakami, Satoshi Odo, Hisao Kamiya

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

The interaction between the multiple lectins (BRA-2, BRA-3) in the coelomic fluid of the acorn barnacle Megabalanus rosa and calcium ions was studied by ultraviolet difference spectroscopy. The association constants for BRA-2 and BRA-3 were 1.4 × 10⁴ M^-1 and 2.2 × 10⁴ M^-1 at pH 8.0, respectively. Multiple lectins inhibited the crystal growth of supersaturated calcium carbonate solution at a lectin concentration of > 0.1 mg/30 ml. Destruction of the carbohydrate-recognition domains of the lectins decreased the inhibitory activities on the crystal growth. Incorporation of BRAs into the crystal was demonstrated using FITC-labeled lectins.

Original language	English
Pages (from-to)	401-409
Number of pages	9
Journal	Comparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume	107
Issue number	3
DOIs	https://doi.org/10.1016/0305-0491(94)90204-6
Publication status	Published - 1994 Mar

Keywords

Calcium carbonate
Lectins
Megabalanus rosa
Recognition domains

ASJC Scopus subject areas

Biochemistry
Physiology
Molecular Biology

Access to Document

10.1016/0305-0491(94)90204-6

Fingerprint Dive into the research topics of 'Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa'. Together they form a unique fingerprint.

Cite this

Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa. / Muramoto, Koji; Yako, Hiroshi; Murakami, Koji; Odo, Satoshi; Kamiya, Hisao.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 107, No. 3, 03.1994, p. 401-409.

Research output: Contribution to journal › Article › peer-review

@article{106217604ba44579bbe297a0be60ab37,

title = "Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa",

abstract = "The interaction between the multiple lectins (BRA-2, BRA-3) in the coelomic fluid of the acorn barnacle Megabalanus rosa and calcium ions was studied by ultraviolet difference spectroscopy. The association constants for BRA-2 and BRA-3 were 1.4 × 104 M-1 and 2.2 × 104 M-1 at pH 8.0, respectively. Multiple lectins inhibited the crystal growth of supersaturated calcium carbonate solution at a lectin concentration of > 0.1 mg/30 ml. Destruction of the carbohydrate-recognition domains of the lectins decreased the inhibitory activities on the crystal growth. Incorporation of BRAs into the crystal was demonstrated using FITC-labeled lectins.",

keywords = "Calcium carbonate, Lectins, Megabalanus rosa, Recognition domains",

author = "Koji Muramoto and Hiroshi Yako and Koji Murakami and Satoshi Odo and Hisao Kamiya",

year = "1994",

month = mar,

doi = "10.1016/0305-0491(94)90204-6",

language = "English",

volume = "107",

pages = "401--409",

journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",

issn = "1096-4959",

number = "3",

}

TY - JOUR

T1 - Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa

AU - Muramoto, Koji

AU - Yako, Hiroshi

AU - Murakami, Koji

AU - Odo, Satoshi

AU - Kamiya, Hisao

PY - 1994/3

Y1 - 1994/3

N2 - The interaction between the multiple lectins (BRA-2, BRA-3) in the coelomic fluid of the acorn barnacle Megabalanus rosa and calcium ions was studied by ultraviolet difference spectroscopy. The association constants for BRA-2 and BRA-3 were 1.4 × 104 M-1 and 2.2 × 104 M-1 at pH 8.0, respectively. Multiple lectins inhibited the crystal growth of supersaturated calcium carbonate solution at a lectin concentration of > 0.1 mg/30 ml. Destruction of the carbohydrate-recognition domains of the lectins decreased the inhibitory activities on the crystal growth. Incorporation of BRAs into the crystal was demonstrated using FITC-labeled lectins.

AB - The interaction between the multiple lectins (BRA-2, BRA-3) in the coelomic fluid of the acorn barnacle Megabalanus rosa and calcium ions was studied by ultraviolet difference spectroscopy. The association constants for BRA-2 and BRA-3 were 1.4 × 104 M-1 and 2.2 × 104 M-1 at pH 8.0, respectively. Multiple lectins inhibited the crystal growth of supersaturated calcium carbonate solution at a lectin concentration of > 0.1 mg/30 ml. Destruction of the carbohydrate-recognition domains of the lectins decreased the inhibitory activities on the crystal growth. Incorporation of BRAs into the crystal was demonstrated using FITC-labeled lectins.

KW - Calcium carbonate

KW - Lectins

KW - Megabalanus rosa

KW - Recognition domains

UR - http://www.scopus.com/inward/record.url?scp=0028218411&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028218411&partnerID=8YFLogxK

U2 - 10.1016/0305-0491(94)90204-6

DO - 10.1016/0305-0491(94)90204-6

M3 - Article

AN - SCOPUS:0028218411

VL - 107

SP - 401

EP - 409

JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

SN - 1096-4959

IS - 3

ER -