Abstract
The interaction between the multiple lectins (BRA-2, BRA-3) in the coelomic fluid of the acorn barnacle Megabalanus rosa and calcium ions was studied by ultraviolet difference spectroscopy. The association constants for BRA-2 and BRA-3 were 1.4 × 104 M-1 and 2.2 × 104 M-1 at pH 8.0, respectively. Multiple lectins inhibited the crystal growth of supersaturated calcium carbonate solution at a lectin concentration of > 0.1 mg/30 ml. Destruction of the carbohydrate-recognition domains of the lectins decreased the inhibitory activities on the crystal growth. Incorporation of BRAs into the crystal was demonstrated using FITC-labeled lectins.
Original language | English |
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Pages (from-to) | 401-409 |
Number of pages | 9 |
Journal | Comparative Biochemistry and Physiology -- Part B: Biochemistry and |
Volume | 107 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1994 Mar |
Externally published | Yes |
Keywords
- Calcium carbonate
- Lectins
- Megabalanus rosa
- Recognition domains
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Molecular Biology