TY - JOUR
T1 - Inhibition of N-Vanillylnonanamide in anaerobic digestion of lipids in food waste
T2 - Microorganisms damage and blocked electron transfer
AU - Yue, Liangchen
AU - Cheng, Jun
AU - Zhang, Haihua
AU - Yuan, Luyun
AU - Hua, Junjie
AU - Dong, Haiquan
AU - Li, Yu you
AU - Zhou, Junhu
N1 - Funding Information:
This study was supported by the National Key Research and Development Program-China ( 2016YFE0117900 ), and Zhejiang Provincial Key Research and Development Program-China ( 2017C04001 ).
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/11/15
Y1 - 2020/11/15
N2 - To study the inhibited degradation metabolism and anaerobic digestion of typical lipids in food waste, an artificially produced capsaicin, N-Vanillylnonanamide, a typical soluble component in waste lipids, was added to a glycerol trioleate anaerobic digestion system. The microorganisms damage and blocked electron transfer caused by N-Vanillylnonanamide during anaerobic digestion were further clarified. Scanning electron microscopy and transmission electron microscopy images demonstrated that N-Vanillylnonanamide (≥4 wt%) structurally damaged microorganisms via cell membrane breakage, which impair their function. N-Vanillylnonanamide inhibited the activities of the key enzyme CoA, AK, F420, and CoM, which are relevant for both degradation metabolism and anaerobic digestion. 16S rRNA analysis showed that dominant bacterial and archaeal communities markedly decreased after anaerobic digestion of glycerol trioleate with N-Vanillylnonanamide (≥4 wt%). For example, the proportion of Methanosarcina decreased from 30 % to 6 %. Current-voltage curves indicated that the electron transfer rate in the community of microorganisms decreased by 99 % from 4.67 × 10−2 to 5.66 × 10−4 s−1 in response to N-Vanillylnonanamide (40 wt%). The methane yield during anaerobic digestion of glycerol trioleate decreased by 84.0 % from 780.21–142.10 mL/g-total volatile solids with N-Vanillylnonanamide (40 wt%).
AB - To study the inhibited degradation metabolism and anaerobic digestion of typical lipids in food waste, an artificially produced capsaicin, N-Vanillylnonanamide, a typical soluble component in waste lipids, was added to a glycerol trioleate anaerobic digestion system. The microorganisms damage and blocked electron transfer caused by N-Vanillylnonanamide during anaerobic digestion were further clarified. Scanning electron microscopy and transmission electron microscopy images demonstrated that N-Vanillylnonanamide (≥4 wt%) structurally damaged microorganisms via cell membrane breakage, which impair their function. N-Vanillylnonanamide inhibited the activities of the key enzyme CoA, AK, F420, and CoM, which are relevant for both degradation metabolism and anaerobic digestion. 16S rRNA analysis showed that dominant bacterial and archaeal communities markedly decreased after anaerobic digestion of glycerol trioleate with N-Vanillylnonanamide (≥4 wt%). For example, the proportion of Methanosarcina decreased from 30 % to 6 %. Current-voltage curves indicated that the electron transfer rate in the community of microorganisms decreased by 99 % from 4.67 × 10−2 to 5.66 × 10−4 s−1 in response to N-Vanillylnonanamide (40 wt%). The methane yield during anaerobic digestion of glycerol trioleate decreased by 84.0 % from 780.21–142.10 mL/g-total volatile solids with N-Vanillylnonanamide (40 wt%).
KW - Anaerobic digestion
KW - Food waste
KW - Glycerol trioleate
KW - Inhibition
KW - N-Vanillylnonanamide
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U2 - 10.1016/j.jhazmat.2020.123098
DO - 10.1016/j.jhazmat.2020.123098
M3 - Article
C2 - 32937719
AN - SCOPUS:85086593231
VL - 399
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
SN - 0304-3894
M1 - 123098
ER -