Inhibition of infection of incoming HIV-1 virus by RNA-cleaving DNA enzyme

Xiaoyan Zhang, Younong Xu, Hong Ling, Toshio Hattori

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Nine different DNA enzymes (DzV3-n, n=1-9) targeting the V3 loop region of HIV-1 HXB2 were synthesized. One of those, DzV3-9, efficiently cleaved the target in the conserved sequence in the RNA transcript in vitro. DzV3-9 was stable in the cells and inhibited replication of both NL432 and SF162 strains in U87 cells expressing CD4 and co-receptors. The inhibitory effect of DNAzyme on incoming HIV-1 was also demonstrated with pseudotype virions generated by NL432-based luciferase reporter genes. Thus, an efficient, stable DNAzyme against a functionally important region of HIV-1 was identified, and it may be useful for prevention of HIV-1 infection. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalFEBS Letters
Volume458
Issue number2
DOIs
Publication statusPublished - 1999 Sep 17

Keywords

  • DNA enzyme
  • Gene therapy
  • Inhibition of human immunodeficiency virus type 1 infection
  • V3 loop

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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