Inhibition of in vitro fertilization by a monoclonal antibody reacting with the zona pellucida of the oviductal egg but not with that of the ovarian egg of the golden hamster

Yasuko Sakai, Yoshihiko Araki, Takao Yamashita, Shoichiro Kurata, Taneaki Oikawa, Masahiko Hiroi, Fujiro Sendo

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27 Citations (Scopus)

Abstract

A glycoprotein designated ZP-0 is secreted from the epithelial cells of the oviduct and is bound to the zonae pellucidae (ZPe) of the oviductal eggs. We have previously established a monoclonal antibody reacting with ZP-0, termed AZPO-8. In the present study, we have examined the effect of AZPO-8 on in vitro fertilization in the hamster. Treatment of the oviductal eggs with AZPO-8 at concentrations of more than 100 μg/ml resulted in: (1) the formation of "zona precipitation" on the outer region of the ZP; (2) inhibition of sperm binding to ZP; (3) the acquisition of resistance to protease treatment. Fertilization was inhibited by AZPO-8 in a dose-dependent manner. These results indicate that a monoclonal antibody (MoAb) to an additional component of ZP derived from the oviduct shows an inhibitory effect on fertilization in vitro.

Original languageEnglish
Pages (from-to)177-189
Number of pages13
JournalJournal of Reproductive Immunology
Volume14
Issue number2
DOIs
Publication statusPublished - 1988 Nov
Externally publishedYes

Keywords

  • hamster
  • in vitro fertilization
  • monoclonal antibody
  • oviduct
  • zona pellucida

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

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