Abstract
Ghrelin and growth hormone secretagogues receptor (GHS-R or ghrelin receptor) have been reported as being one of the factors of adipogenesis in adipocytes. To investigate the involvement of ghrelin and GHS-R in adipocytes, the effect of the GHS-R antagonist, [D-Lys-3]-GHRP-6 (His-D-Trp-D-Lys-Trp-D-Phe- Lys-NH 2), on the process of adipogenesis in ovine and rat adipocytes was evaluated. [D-Lys-3]-GHRP-6 (10 -7 mol/L) significantly inhibited adipogenic differentiation of ovine and rat preadipocytes prepared from adipose tissues. The level of peroxisome proliferator activated receptor (PPAR)-γ2 mRNA, an adipogenic marker, was decreased during the differentiation of adipocytes treated with [D-Lys-3]-GHRP-6 for 10 days. Ghrelin stimulated adipogenesis, also causing an increment of glycerol-3-phosphate dehydrogenase and upregulation of PPAR-γ2. Furthermore, the antilipolytic effect of ghrelin was attenuated by treatment with [D-Lys-3]-GHRP-6 in both types of isolated adipocytes. Overall, the results of the present study highlight that GHS-R in adipogenesis can be blocked by treatment with [D-Lys-3]-GHRP-6.
Original language | English |
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Pages (from-to) | 381-386 |
Number of pages | 6 |
Journal | Animal Science Journal |
Volume | 76 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2005 Aug |
Externally published | Yes |
Keywords
- Adipogenesis
- Growth hormone secretagogues receptor
- [D-Lys-3]-GHRP-6
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)