Inhibition of growth hormone secretagogue receptor antagonist, [D-Lys-3]-GHRP-6, in adipogenesis of ovine and rat adipocytes

Sang Gun Roh, Yeon Hee Hong, Daisuke Hishikawa, Hiroaki Tsuzuki, Hisae Miyahara, Yukihiko Nishimura, Chizu Goto, Yasuki Suzuki, Ki Choon Choi, Hong Gu Lee, Shinichi Sasaki

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Ghrelin and growth hormone secretagogues receptor (GHS-R or ghrelin receptor) have been reported as being one of the factors of adipogenesis in adipocytes. To investigate the involvement of ghrelin and GHS-R in adipocytes, the effect of the GHS-R antagonist, [D-Lys-3]-GHRP-6 (His-D-Trp-D-Lys-Trp-D-Phe- Lys-NH 2), on the process of adipogenesis in ovine and rat adipocytes was evaluated. [D-Lys-3]-GHRP-6 (10 -7 mol/L) significantly inhibited adipogenic differentiation of ovine and rat preadipocytes prepared from adipose tissues. The level of peroxisome proliferator activated receptor (PPAR)-γ2 mRNA, an adipogenic marker, was decreased during the differentiation of adipocytes treated with [D-Lys-3]-GHRP-6 for 10 days. Ghrelin stimulated adipogenesis, also causing an increment of glycerol-3-phosphate dehydrogenase and upregulation of PPAR-γ2. Furthermore, the antilipolytic effect of ghrelin was attenuated by treatment with [D-Lys-3]-GHRP-6 in both types of isolated adipocytes. Overall, the results of the present study highlight that GHS-R in adipogenesis can be blocked by treatment with [D-Lys-3]-GHRP-6.

Original languageEnglish
Pages (from-to)381-386
Number of pages6
JournalAnimal Science Journal
Volume76
Issue number4
DOIs
Publication statusPublished - 2005 Aug
Externally publishedYes

Keywords

  • Adipogenesis
  • Growth hormone secretagogues receptor
  • [D-Lys-3]-GHRP-6

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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