Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis

Keisuke Ikawa; Ayaka Satou; Mitsuko Fukuhara; Shigeru Matsumura; Naoyuki Sugiyama; Hidemasa Goto; Mitsunori Fukuda; Masaki Inagaki; Yasushi Ishihama; Fumiko Toyoshima

doi:10.4161/cc.26866

Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis

Keisuke Ikawa, Ayaka Satou, Mitsuko Fukuhara, Shigeru Matsumura, Naoyuki Sugiyama, Hidemasa Goto, Mitsunori Fukuda, Masaki Inagaki, Yasushi Ishihama, Fumiko Toyoshima

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Endocytic vesicle fusion is inhibited during mitosis, but the molecular pathways that mediate the inhibition remain unclear. Here we uncovered an essential role of Polo-like kinase 1 (Plk1) in this mechanism. Phosphoproteomic analysis revealed that Plk1 phosphorylates the intermediate filament protein vimentin on Ser459, which is dispensable for its filament formation but is necessary for the inhibition of endocytic vesicle fusion in mitosis. Furthermore, this mechanism is required for integrin trafficking toward the cleavage furrow during cytokinesis. Our results thus identify a novel mechanism for fusion inhibition in mitosis and implicate its role in vesicle trafficking after anaphase onset.

Original language	English
Pages (from-to)	126-137
Number of pages	12
Journal	Cell Cycle
Volume	13
Issue number	1
DOIs	https://doi.org/10.4161/cc.26866
Publication status	Published - 2014 Jan 1

Keywords

Endosome
Mitosis
Plk1
Vesicle fusion
Vimentin

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

Access to Document

10.4161/cc.26866

Cite this

Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis. / Ikawa, Keisuke; Satou, Ayaka; Fukuhara, Mitsuko; Matsumura, Shigeru; Sugiyama, Naoyuki; Goto, Hidemasa; Fukuda, Mitsunori; Inagaki, Masaki; Ishihama, Yasushi; Toyoshima, Fumiko.

In: Cell Cycle, Vol. 13, No. 1, 01.01.2014, p. 126-137.

Research output: Contribution to journal › Article › peer-review

@article{b15a4d2afd9041e5ab2b5c62c3b74b28,

title = "Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis",

abstract = "Endocytic vesicle fusion is inhibited during mitosis, but the molecular pathways that mediate the inhibition remain unclear. Here we uncovered an essential role of Polo-like kinase 1 (Plk1) in this mechanism. Phosphoproteomic analysis revealed that Plk1 phosphorylates the intermediate filament protein vimentin on Ser459, which is dispensable for its filament formation but is necessary for the inhibition of endocytic vesicle fusion in mitosis. Furthermore, this mechanism is required for integrin trafficking toward the cleavage furrow during cytokinesis. Our results thus identify a novel mechanism for fusion inhibition in mitosis and implicate its role in vesicle trafficking after anaphase onset.",

keywords = "Endosome, Mitosis, Plk1, Vesicle fusion, Vimentin",

author = "Keisuke Ikawa and Ayaka Satou and Mitsuko Fukuhara and Shigeru Matsumura and Naoyuki Sugiyama and Hidemasa Goto and Mitsunori Fukuda and Masaki Inagaki and Yasushi Ishihama and Fumiko Toyoshima",

year = "2014",

month = jan,

day = "1",

doi = "10.4161/cc.26866",

language = "English",

volume = "13",

pages = "126--137",

journal = "Cell Cycle",

issn = "1538-4101",

publisher = "Landes Bioscience",

number = "1",

}

TY - JOUR

T1 - Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis

AU - Ikawa, Keisuke

AU - Satou, Ayaka

AU - Fukuhara, Mitsuko

AU - Matsumura, Shigeru

AU - Sugiyama, Naoyuki

AU - Goto, Hidemasa

AU - Fukuda, Mitsunori

AU - Inagaki, Masaki

AU - Ishihama, Yasushi

AU - Toyoshima, Fumiko

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Endocytic vesicle fusion is inhibited during mitosis, but the molecular pathways that mediate the inhibition remain unclear. Here we uncovered an essential role of Polo-like kinase 1 (Plk1) in this mechanism. Phosphoproteomic analysis revealed that Plk1 phosphorylates the intermediate filament protein vimentin on Ser459, which is dispensable for its filament formation but is necessary for the inhibition of endocytic vesicle fusion in mitosis. Furthermore, this mechanism is required for integrin trafficking toward the cleavage furrow during cytokinesis. Our results thus identify a novel mechanism for fusion inhibition in mitosis and implicate its role in vesicle trafficking after anaphase onset.

AB - Endocytic vesicle fusion is inhibited during mitosis, but the molecular pathways that mediate the inhibition remain unclear. Here we uncovered an essential role of Polo-like kinase 1 (Plk1) in this mechanism. Phosphoproteomic analysis revealed that Plk1 phosphorylates the intermediate filament protein vimentin on Ser459, which is dispensable for its filament formation but is necessary for the inhibition of endocytic vesicle fusion in mitosis. Furthermore, this mechanism is required for integrin trafficking toward the cleavage furrow during cytokinesis. Our results thus identify a novel mechanism for fusion inhibition in mitosis and implicate its role in vesicle trafficking after anaphase onset.

KW - Endosome

KW - Mitosis

KW - Plk1

KW - Vesicle fusion

KW - Vimentin

UR - http://www.scopus.com/inward/record.url?scp=84892606182&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892606182&partnerID=8YFLogxK

U2 - 10.4161/cc.26866

DO - 10.4161/cc.26866

M3 - Article

C2 - 24196446

AN - SCOPUS:84892606182

VL - 13

SP - 126

EP - 137

JO - Cell Cycle

JF - Cell Cycle

SN - 1538-4101

IS - 1

ER -

Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this