Abstract
Endocytic vesicle fusion is inhibited during mitosis, but the molecular pathways that mediate the inhibition remain unclear. Here we uncovered an essential role of Polo-like kinase 1 (Plk1) in this mechanism. Phosphoproteomic analysis revealed that Plk1 phosphorylates the intermediate filament protein vimentin on Ser459, which is dispensable for its filament formation but is necessary for the inhibition of endocytic vesicle fusion in mitosis. Furthermore, this mechanism is required for integrin trafficking toward the cleavage furrow during cytokinesis. Our results thus identify a novel mechanism for fusion inhibition in mitosis and implicate its role in vesicle trafficking after anaphase onset.
Original language | English |
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Pages (from-to) | 126-137 |
Number of pages | 12 |
Journal | Cell Cycle |
Volume | 13 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2014 Jan 1 |
Keywords
- Endosome
- Mitosis
- Plk1
- Vesicle fusion
- Vimentin
ASJC Scopus subject areas
- Molecular Biology
- Developmental Biology
- Cell Biology