Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis

Keisuke Ikawa, Ayaka Satou, Mitsuko Fukuhara, Shigeru Matsumura, Naoyuki Sugiyama, Hidemasa Goto, Mitsunori Fukuda, Masaki Inagaki, Yasushi Ishihama, Fumiko Toyoshima

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Endocytic vesicle fusion is inhibited during mitosis, but the molecular pathways that mediate the inhibition remain unclear. Here we uncovered an essential role of Polo-like kinase 1 (Plk1) in this mechanism. Phosphoproteomic analysis revealed that Plk1 phosphorylates the intermediate filament protein vimentin on Ser459, which is dispensable for its filament formation but is necessary for the inhibition of endocytic vesicle fusion in mitosis. Furthermore, this mechanism is required for integrin trafficking toward the cleavage furrow during cytokinesis. Our results thus identify a novel mechanism for fusion inhibition in mitosis and implicate its role in vesicle trafficking after anaphase onset.

    Original languageEnglish
    Pages (from-to)126-137
    Number of pages12
    JournalCell Cycle
    Volume13
    Issue number1
    DOIs
    Publication statusPublished - 2014 Jan 1

    Keywords

    • Endosome
    • Mitosis
    • Plk1
    • Vesicle fusion
    • Vimentin

    ASJC Scopus subject areas

    • Molecular Biology
    • Developmental Biology
    • Cell Biology

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