Inhibition by retinoids of antigen-induced IL-4 production in rat mast cell line RBL-2H3

Noriyasu Hirasawa, Hiroyuki Kagechika, Koichi Shudo, Kazuo Ohuchi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The retinoic acid receptor (RAR) agonists, Re80 and Am80, partially inhibited the antigen-induced IL-4 production by rat mast cell line RBL-2H3 in a concentration-dependent manner (0.1 to 1000 nM). Both Re80 and Am80 also reduced the antigen-induced increase in IL-4 mRNA levels. The RAR antagonist LE540 at 4 μM reversed Re80 (100 nM)- and Am80 (100 nM)-induced inhibition of IL-4 production. The retinoid X receptor agonist HX600 (1 μM) by itself did not affect IL-4 production, but enhanced the inhibitory effect of Re80 (10 nM) and of Am80 (10 nM). Cyclosporin A suppressed the antigen-induced IL-4 production almost completely at 0.3 μM. These findings indicated that the antigen-induced IL-4 production by RBL-2H3 cells is partially inhibited by retinoids via RAR-dependent mechanisms.

Original languageEnglish
Pages (from-to)1287-1294
Number of pages8
JournalLife Sciences
Volume68
Issue number11
DOIs
Publication statusPublished - 2001 Feb 2

Keywords

  • Cyclosporin A
  • IL-4
  • Mast cell
  • Retinoid

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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