Inhibition by polyphenolic phytochemicals and sulfurous compounds of the formation of 8-chloroguanosine mediated by hypochlorous acid, human myeloperoxidase, and activated human neutrophils

Toshiki Nakano, Mitsuharu Masuda, Toshinori Suzuki, Hiroshi Ohshima

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    Hypochlorous acid (HOCl) produced by myeloperoxidase (MPO) of activated neutrophils can react with nucleic acid bases to form chlorinated nucleosides such as 8-chloroguanosine (Cl-Guo). Chlorination is enhanced by nicotine. We investigated the effects of various natural antioxidants including polyphenolic phytochemicals on the formation of Cl-Guo by HOCl in the presence and the absence of nicotine. Polyphenols, including catechins, curcumin, resveratrol, silibinin, and sulfurous compound α-lipoic acid, were found to inhibit both HOCl- and human MPO-induced Cl-Guo formation dose-dependently. Among the test compounds, (-)-epigallocatechin gallate (EGCG) showed the strongest inhibitory effect. Cl-Guo formation, mediated by activated human neutrophils in the presence of nicotine, was inhibited by EGCG, silibinin, and α-lipoic acid. These results suggest that polyphenols and sulfurous compounds have the potential to inhibit the induction of nucleobase damage mediated by chlorination, with possible application to reducing DNA damage associated with inflammation and cigarettesmoke inhalation.

    Original languageEnglish
    Pages (from-to)2208-2213
    Number of pages6
    JournalBioscience, Biotechnology and Biochemistry
    Volume76
    Issue number12
    DOIs
    Publication statusPublished - 2012

    Keywords

    • Chlorination
    • Hypochlorous acid
    • Neutrophils
    • Nicotine
    • Polyphenolic phytochemicals

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

    Fingerprint

    Dive into the research topics of 'Inhibition by polyphenolic phytochemicals and sulfurous compounds of the formation of 8-chloroguanosine mediated by hypochlorous acid, human myeloperoxidase, and activated human neutrophils'. Together they form a unique fingerprint.

    Cite this