Inhibition by gossypol of tumor promoter-induced arachidonic acid metabolism in rat peritoneal macrophages

Kazuo Ohuchi, Masako Watanabe, Noriyasu Hirasawa, Susumu Tsurufuji, Tsuneo Ozeki, Hirota Fujiki

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Rat peritoneal macrophages were prelabeled with [3H]arachidonic acid. The release of radioactivity into the medium was increased by treatment with TPA-type tumor promoters, such as TPA, teleocidin and aplysiatoxin, and the non-TPA-type tumor promoter, thapsigargin. Gossypol, at concentrations of 3 and 10 μM, inhibited the release of radioactivity stimulated by both types of tumor promoter, although the mechanism of stimulation of arachidonic acid metabolism is different in the two types of tumor promoter. Stimulation of prostaglandin E2 production by these tumor promoters was also inhibited by treatment with gossypol. Calcium ionophore A23187-stimulated release of radioactivity and prostaglandin E2 production were also inhibited by gossypol treatment. The mechanism of inhibition by gossypol of prostaglandin E2 production is discussed.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalBBA - Molecular Cell Research
Volume971
Issue number1
DOIs
Publication statusPublished - 1988 Aug 19

Keywords

  • (Rat peritoneal macrophage)
  • Arachidonic acid metabolism
  • Gossypol
  • Prostaglandin E
  • Tumor promotor

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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