Inheritance of microsatellite DNA markers in the pacific abalone Haliotis discus hannai

Qi Li, Choulji Park, Toshimasa Kobayashi, Akihiro Kijima

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Microsatellite markers have been developed for a variety of abalones, and locus-specific homozygote excesses at population level have been recorded for microsatellite loci. To ascertain whether null alleles exist at microsatellite loci in the Pacific abalone, we studied the mode of inheritance of 7 microsatellite loci in 4 families with a reciprocal cross of 2 females x 2 males. All loci segregated codominantly, but only 3 loci (Hdh1321, Hdh78, and Hdd108C) conformed to Mendelian segregation and can be used for parental analysis and population genetic studies. When null alleles were considered, 2 loci (Hdh1761 and Hdh1457) confirmed Mendelian expectations in all families, while the remaining 2 loci (Hdd114B and Hdd229) showed deviation from Mendelian segregation in at least one family even though null alleles were considered. These results indicated the need to test the inheritance pattern for microsatellite markers in abalones before using them for population genetic or parentage analysis.

Original languageEnglish
Pages (from-to)331-338
Number of pages8
JournalMarine Biotechnology
Volume5
Issue number4
DOIs
Publication statusPublished - 2003 Jul 1

Keywords

  • Inheritance
  • Microsatellites
  • Pacific abaloneand Haliotis discus hannai

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

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